Histone H2B antibody

Catalog No. ABIN317616

Product Details anti-Histone H2B Antibody

Target: Histone H2B

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: Western Blot: 1/500-1/1000. ELISA: 1/5000-1/1000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Histone H2B

Target: Histone H2B

Abstract: Histone H2B Products

Background: In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B H3 and H4 and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. Histone H1 is required for the condensation of nucleosome chains into higher order structures. Phosporylation of Histone H1 is thought be involved in this process, although the exact nature of this role has yet to be elucidated. Evidence suggests that Histone H1 is a part of a general repressor mechanism for stable repression of transcription, but it can also activate transcription of specific genes.Synonyms: H2B Histone Family, HIST1H2B

Molecular Weight: approx. 18 kDa