CD14 antibody (PE)
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- Target See all CD14 Antibodies
- CD14
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Reactivity
- Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD14 antibody is conjugated to PE
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Application
- Flow Cytometry (FACS)
- Characteristics
- Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein
- Purification
- Purified IgG prepared by affinity chromatography on Protein G
- Immunogen
- Recombinant mouse CD14
- Clone
- BiG53
- Isotype
- IgG1
- Top Product
- Discover our top product CD14 Primary Antibody
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- Application Notes
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Flow Cytometry: Use 10 μL of neat antibody to label 1x10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore with 1 mL distilled water
- Buffer
- PBS pH 7.4 containing 0.09 % Sodium Azide and 1 % BSA
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
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Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!
Shelf life: one year from despatch. - Expiry Date
- 12 months
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- Target
- CD14
- Alternative Name
- CD14 (CD14 Products)
- Background
- CD14 is a 55kD a glycophosphoinositol-linked cell surface protein that also exists in a soluble form. Expression of CD14 is restricted to the myeloid lineage and is predominantly expressed on peripheral blood monocytes, weakly expressed on granulocytes and absent on myeloid progenitors. The CD14 Molecule acts as a pattern recognition receptor, which binds lipopolysaccharide (LPS) and other microbial products such as lipoteichoic acid, lipoproteins and peptidoglycan. Binding of LPS to CD14 mediates the innate immune response to LPS and requires the Toll-like receptor-4 (TLR4)/MD-2 complex for effective signalling.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein
- UniProt
- P10810
- Pathways
- TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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