The antibody recognizes human interferon regulatory factor 3 (IRF3), a 55 kDa constitutively expressed member of the IRF family. Other species not tested.
Characteristics
Synonyms: Interferon regulatory factor 3, IRF-3
Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Recombinant human IRF3 (108-166 aa) purified from E. coli
ELISA. Western blot (1: 1,000 - 1: 3,000), recommended starting dilution is 1: 2,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Members of the Interferon regulatory factor (IRF) family regulate gene expression critical to immune response, hemopoiesis, and proliferation. IRF-3 is distinct from other family members. Its transcriptional activity is regulated solely by posttranslational modifications. It plays a crucial role in activation of innate immunity and inflammation in response to viral infection. IRF3 accumulates in an inactive form in the cytoplasm and then translocates to the nucleus as a homodimer, following stimuli-induced phosphorylation of specific serine/threonine residues. Association of the homodimer with p300/CBP (CREBB binding protein) forms dsRNA-activated factor 1 (DRAF1), which then activates the transcription of interferon alpha and beta, along with other interferon-induced genes.Synonyms: IRF-3, Interferon regulatory factor 3