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CD4 antibody (FITC)

CD4 Reactivity: Mouse FACS Host: Rat Monoclonal RM4-5 FITC

Catalog No. ABIN317080

Datasheet as PDF

Target See all CD4 Antibodies

CD4
Reactivity
334

199

117

48

24

20

19

18

18

12

10

10

10

10

10

9

9

9

8

5

4

3

1

1

1

1

1
Mouse
Host
341

153

109

3

3

1
Rat
Compatible Secondaries
Clonality
463

138

6
Monoclonal
Conjugate
215

72

63

45

33

13

12

11

9

9

9

8

5

4

4

4

4

4

4

4

4

4

4

4

4

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1
This CD4 antibody is conjugated to FITC
Application
488

195

128

108

94

76

69

42

39

34

33

28

10

9

9

7

6

6

5

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS)

Specificity

This antibody detects CD4, a 55 kDa protein also known as Ly-4 and L3T4. This antibody Clone RM4-5 is useful for in vitro blocking of ligand binding, as well as in vitro CD4+ T cell depletions.

Characteristics

Synonyms: T4/Leu-3, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

Purification

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen

BALB/c mouse thymocytes.

Clone

RM4-5

Isotype

IgG2a

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anti-CD4 (CD4) (AA 385-457) antibody
CD4 Reactivity: Human, Mouse, Rat, Chicken, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 Reactivity: Human, Mouse, Rat, Chicken, Monkey, Dog, Pig, Cat, Cow, Rabbit, Sheep, Guinea Pig, Goat, Horse, Avian, Donkey, Hamster WB Host: Goat Polyclonal unconjugated

anti-CD4 (CD4) (AA 231-330) antibody
CD4 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody
CD4 Reactivity: Human FACS, WB, CyTOF Host: Mouse Monoclonal MEM-241 unconjugated

anti-CD4 (CD4) (AA 300-400) antibody
CD4 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 Reactivity: Mouse FACS, IP, IHC (fro), ICC, Func Host: Rat Monoclonal GK1-5 unconjugated

anti-CD4 (CD4) (AA 301-400) antibody
CD4 Reactivity: Human, Mouse, Rat FACS, WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody (FITC)
CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 FITC

anti-CD4 (CD4) (N-Term) antibody (PE)
CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 PE

anti-CD4 (CD4) antibody (PE)
CD4 Reactivity: Rat FACS Host: Mouse Monoclonal OX-35 PE

Application Notes

Flow Cytometry. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing lowaffinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

0.5 mg/mL

Buffer

PBS containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.

Expiry Date

12 months
Target

CD4

Alternative Name

CD4 (CD4 Products)

Background

CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+CD25+), which play a key role in the maintenance of self tolerance.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Gene ID

12504

UniProt

P06332

Pathways

TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events