MCM8 antibody (C-Term)

Catalog No. ABIN309831

Product Details anti-MCM8 Antibody

Target: MCM8 (Minichromosome Maintenance Deficient 8 (MCM8))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse, Cow, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio), Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: IRLTEARARL ELREEATKED AEDIVEIMKY SMLGTYSDEF GNLDFERSQH

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against MCM8. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human MCM8

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 840 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-MCM8 antibody (ABIN309831)

Ono, Kumai, Koizumi, Nishikawa, Kobayashi, Tadokoro: "Overexpression of heat shock protein 27 in squamous cell carcinoma of the uterine cervix: a proteomic analysis using archival formalin-fixed, paraffin-embedded tissues." in: Human pathology, Vol. 40, Issue 1, pp. 41-9, (2008) (PubMed).

Target Details for MCM8

Target: MCM8 (Minichromosome Maintenance Deficient 8 (MCM8))

Alternative Name: MCM8 (MCM8 Products)

Synonyms: C20orf154 antibody, dJ967N21.5 antibody, 5730432L01Rik antibody, minichromosome maintenance 8 homologous recombination repair factor antibody, minichromosome maintenance 8 homologous recombination repair factor L homeolog antibody, MCM8 antibody, Mcm8 antibody, mcm8.L antibody

Background: MCM8 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein contains the central domain that is conserved among the MCM proteins. This protein has been shown to co-immunoprecipitate with MCM4, 6 and 7, which suggests that it may interact with other MCM proteins and play a role in DNA replication. Alternatively spliced transcript variants encoding distinct isoforms have been described.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein contains the central domain that is conserved among the MCM proteins. This protein has been shown to co-immunoprecipitate with MCM4, 6 and 7, which suggests that it may interact with other MCM proteins and play a role in DNA replication. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Alias Symbols: C20orf154, MGC119522, MGC119523, MGC12866, MGC4816, REC, dJ967N21.5
Protein Interaction Partner: MCMBP, MCM4, MCM6, MCM7, ORC2, CDC6,
Protein Size: 840

Molecular Weight: 94 kDa

Gene ID: 84515

NCBI Accession: NM_032485, NP_115874

UniProt: Q9UJA3

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA