Peroxiredoxin 1 antibody (Internal Region)

Catalog No. ABIN308466

Product Details anti-Peroxiredoxin 1 Antibody

Target: Peroxiredoxin 1 (PRDX1)

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Peroxiredoxin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: PRDX1

Sequence: SDPKRTIAQD YG

Specificity: Reported variants represent identical protein (NP_002565.1, NP_859047.1, NP_859048.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: C-SDPKRTIAQDYG

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 24 kDa band observed in lysates of cell line HEK293, A431 and HepG2 (calculated MW of 22.1 kDa according to NP_859048.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Peroxiredoxin 1

Target: Peroxiredoxin 1 (PRDX1)

Alternative Name: PRDX1 (PRDX1 Products)

Background: PRDX1, peroxiredoxin 1, MSP23, NKEFA, PAG, PAGA, PAGB, PRX1, PRXI, TDPX2, natural killer-enhancing factor A, proliferation-associated gene A, thioredoxin-dependent peroxide reductase 2

Gene ID: 5052, 18477, 117254

NCBI Accession: NP_859048

Pathways: p53 Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events