SAR1A antibody (Internal Region)

Catalog No. ABIN308431

Product Details anti-SAR1A Antibody

Target: SAR1A (SAR1 Homolog A (SAR1A))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This SAR1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: SAR1

Sequence: HSRLVESKVE LNA

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-HSRLVESKVELNA, from the internal region of the protein sequence according to NP_064535.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Skin and Small Intestine. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx. 23 kDa band observed in lysates of NIH3T3 (calculated MW of 22.4 kDa according to Human NP_064535.1 and to Mouse NP_033146.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SAR1A

Target: SAR1A (SAR1 Homolog A (SAR1A))

Alternative Name: SAR1A (SAR1A Products)

Background: SAR1A, SAR1 gene homolog A (S. cerevisiae), SAR1, SARA1, Sara, masra2, OTTHUMP00000060603, SAR1a gene homolog 1

Gene ID: 56681, 20224, 361842

NCBI Accession: NP_064535