NCKAP1L antibody (Internal Region)

Catalog No. ABIN308428

Product Details anti-NCKAP1L Antibody

Target: NCKAP1L (NCK-Associated Protein 1-Like (NCKAP1L))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NCKAP1L antibody is un-conjugated

Application: ELISA

Purpose: HEM1

Sequence: RHTENVTKTK TPE

Cross-Reactivity: Dog, Horse, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Recent

Immunogen: Peptide with sequence C-RHTENVTKTKTPE, from the internal region of the protein sequence according to NP_005328.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments gave an approx. 50 kDa band in Human Tonsil and Thymus lysates after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NCKAP1L

Target: NCKAP1L (NCK-Associated Protein 1-Like (NCKAP1L))

Alternative Name: NCKAP1L (NCKAP1L Products)

Synonyms: HEM1 antibody, 4930568P13Rik antibody, AI463083 antibody, Hem1 antibody, Hemp1 antibody, NCK associated protein 1 like antibody, zgc:172352 antibody, nck-associated protein 1-like antibody, NCK-associated protein 1-like antibody, NCKAP1L antibody, zgc:172352 antibody, LOC100030968 antibody, nckap1l antibody, LOC100618382 antibody, LOC100639088 antibody, Nckap1l antibody

Background: NCKAP1L, NCK-associated protein 1-like, HEM1, hematopoietic protein 1, membrane-associated protein hem-1

Gene ID: 3071, 105855, 315348

NCBI Accession: NP_005328

Pathways: Regulation of Actin Filament Polymerization