ERN1 antibody (Internal Region)

Catalog No. ABIN308413

Product Details anti-ERN1 Antibody

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ERN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Purpose: ERN1 / IRE1a

Sequence: PNAHGKIKAM ISD

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-PNAHGKIKAMISD, from the internal region of the protein sequence according to NP_001424.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 110 kDa band observed in Human Pancreas and Duodenum lysates (calculated MW of 110 kDa according to NP_001424.3). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ERN1

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Alternative Name: ERN1 (ERN1 Products)

Synonyms: IRE1 antibody, IRE1P antibody, IRE1a antibody, hIRE1p antibody, 9030414B18Rik antibody, AI225830 antibody, C85377 antibody, Ire1a antibody, Ire1alpha antibody, Ire1p antibody, RGD1559716 antibody, Ern1 antibody, Ire1 antibody, Ire1b antibody, sb:eu1005 antibody, zgc:109932 antibody, endoplasmic reticulum to nucleus signaling 1 antibody, endoplasmic reticulum to nucleus signaling 2 antibody, endoplasmic reticulum (ER) to nucleus signalling 1 antibody, serine/threonine-protein kinase/endoribonuclease IRE1 antibody, endoplasmic reticulum (ER) to nucleus signalling 2 antibody, ERN1 antibody, ERN2 antibody, Ern1 antibody, LOC524719 antibody, Ern2 antibody, ern1 antibody

Background: ERN1, IRE1, endoplasmic reticulum to nucleus signaling 1, FLJ30999, IRE1P, MGC163277, MGC163279, ER to nucleus signalling 1, endoplasmic reticulum to nucleus signalling 1, inositol-requiring 1, protein kinase/endoribonuclease, IRE1a, IRE1-alpha

Gene ID: 2081, 78943, 498013

NCBI Accession: NP_001424

Pathways: ER-Nucleus Signaling, Unfolded Protein Response