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ERN1 antibody (Internal Region)

Verified ERN1 Reactivity: Human WB, ELISA, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN308413
  • Target See all ERN1 Antibodies
    ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))
    Binding Specificity
    • 16
    • 16
    • 6
    • 6
    • 5
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 71
    • 51
    • 45
    • 16
    • 1
    Human
    Host
    • 70
    • 5
    • 3
    Goat
    Clonality
    • 70
    • 8
    Polyclonal
    Conjugate
    • 34
    • 8
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This ERN1 antibody is un-conjugated
    Application
    • 73
    • 37
    • 29
    • 26
    • 26
    • 10
    • 10
    • 10
    • 9
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Flow Cytometry (FACS)
    Purpose
    ERN1 / IRE1a
    Sequence
    PNAHGKIKAM ISD
    Cross-Reactivity
    Cow, Dog, Human, Mouse, Pig, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-PNAHGKIKAMISD, from the internal region of the protein sequence according to NP_001424.3.
    Isotype
    IgG
    Top Product
    Discover our top product ERN1 Primary Antibody
  • Application Notes

    Western Blot: Approx 110 kDa band observed in Human Pancreas and Duodenum lysates (calculated MW of 110 kDa according to NP_001424.3). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Comment

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))
    Alternative Name
    ERN1 (ERN1 Products)
    Background
    ERN1, IRE1, endoplasmic reticulum to nucleus signaling 1, FLJ30999, IRE1P, MGC163277, MGC163279, ER to nucleus signalling 1, endoplasmic reticulum to nucleus signalling 1, inositol-requiring 1, protein kinase/endoribonuclease, IRE1a, IRE1-alpha
    Gene ID
    2081, 78943, 498013
    NCBI Accession
    NP_001424
    Pathways
    ER-Nucleus Signaling, Unfolded Protein Response
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