MARCH10 antibody (Internal Region)
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- Target See all MARCH10 Antibodies
- MARCH10 (Membrane-Associated Ring Finger (C3HC4) 10, E3 Ubiquitin Protein Ligase (MARCH10))
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Binding Specificity
- Internal Region
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Reactivity
- Human, Rat
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This MARCH10 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Purpose
- MARCH10
- Sequence
- SRNYPQPRTE E
- Specificity
- This antibody is expected to recognise both reported isoforms (NP_001094345.1, NP_689811.2), which are identical.
- Cross-Reactivity
- Cow, Human, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-SRNYPQPRTEE, from the internal region of the protein sequence according to NP_001094345.1, NP_689811.2.
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 5 μg/mL.
Western Blot: Approx. 90 kDa band observed in Rat Testis lysates (calculated MW of 90.5 kDa according to Human NP_689811.2 and of 87.9 kDa according to Mouse NP_766156.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour. An additiona
Peptide ELISA: antibody detection limit dilution 1:8000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- MARCH10 (Membrane-Associated Ring Finger (C3HC4) 10, E3 Ubiquitin Protein Ligase (MARCH10))
- Alternative Name
- MARCH10 (MARCH10 Products)
- Synonyms
- 4933417C16Rik antibody, AV039223 antibody, OTTMUSG00000002847 antibody, Rnf190 antibody, MARCH-X antibody, RGD1311692 antibody, RNF190 antibody, membrane associated ring-CH-type finger 10 antibody, membrane-associated ring finger (C3HC4) 10 antibody, MARCH10 antibody, March10 antibody
- Background
- MARCH10, membrane-associated ring finger (C3HC4) 10 , FLJ35757, MARCH-X, RNF190, ring finger protein 190
- Gene ID
- 162333, 303596
- NCBI Accession
- NP_001094345, NP_689811
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