EBF1 antibody (Internal Region)

Catalog No. ABIN308389

Product Details anti-EBF1 Antibody

Target: EBF1 (Early B-Cell Factor 1 (EBF1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This EBF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Purpose: EBF1 / COE1

Sequence: HNQLPALANT SV

Specificity: This antibody is expected not to cross-react with EBF2, EBF3 and EBF4.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-HNQLPALANTSV, from the internal region of the protein sequence according to NP_076870.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 75 kDa band observed in Human Adipose lysates (calculated MW of 64.5 kDa according to NP_076870.1). Recommended concentration: 0.3-0.1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunoprecipitation: Anonymous customer used this product successfully in IP from induced 3T3-L1 expressing FLAG-EBF1.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for EBF1

Target: EBF1 (Early B-Cell Factor 1 (EBF1))

Alternative Name: EBF1 (EBF1 Products)

Synonyms: EBF1 antibody, 9830166E18Rik antibody, AW990631 antibody, Ebf antibody, O/E-1 antibody, Olf-1 antibody, Olf1 antibody, COE1 antibody, EBF antibody, OLF1 antibody, early B-cell factor 1 antibody, early B cell factor 1 antibody, EBF1 antibody, Ebf1 antibody

Background: EBF1, early B-cell factor 1 , COE1, EBF, O/E-1, OLF1 , Collier, Olf and EBF transcription factor 1, early B-cell factor olfactory neuronal transcription factor 1

Gene ID: 1879, 13591, 116543

NCBI Accession: NP_076870