5-Methylcytidine antibody

Catalog No. ABIN308218

Product Details anti-5-Methylcytidine Antibody

Target: 5-Methylcytidine

Host: Mouse

Clonality: Monoclonal

Conjugate: This 5-Methylcytidine antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Specificity: Modified base 5-methylcytidine

Purification: Purified IgG from culture supernatant by Affinity chromatography on Protein A ceramic hyper D F - Filtration on 0.22 µm.

Clone: 33D3

Isotype: IgG1

Application Details

Application Notes: MeDIP: Used 5ul in 200ul reaction with 2ug digested genomic DNA from Drosophila. Flow Cyt: Use 10ul of working dilution to label 1000000 cells in 100ul. ICC/IF: Used at a dilution of 1/200 for 16 hrs. IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Sodium citrate buffer pH6.0 is recommended for this purpose. IHC-Fr: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

Comment:

For research use only

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml

Buffer: Phosphate Buffered Saline 10 mM – NaCl 0.15 M – pH 7.4

Storage: -20 °C

References for anti-5-Methylcytidine antibody (ABIN308218)

Ohta, Sakaide, Wakayama: "Functional analysis of male mouse haploid germ cells of various differentiation stages: early and late round spermatids are functionally equivalent in producing progeny." in: Biology of reproduction, Vol. 80, Issue 3, pp. 511-7, (2009) (PubMed).

Xing, Shi, Le, Lee, Silver-Morse, Li: "Evidence for transgenerational transmission of epigenetic tumor susceptibility in Drosophila." in: PLoS genetics, Vol. 3, Issue 9, pp. 1598-606, (2007) (PubMed).

Target Details for 5-Methylcytidine

Target: 5-Methylcytidine

Abstract: 5-Methylcytidine Products

Target Type: Chemical

Background: DNA methylation is a DNA modification process, which is involved in the control of gene expression. The 5-Methylcytidine antibody specifically binds the modified base 5-methylcytidine (5-MeCyd) found in DNA of plants and vertebrates.