MBP antibody (AA 130-136)

Catalog No. ABIN307190

Product Details anti-MBP Antibody

Target: MBP (Myelin Basic Protein (MBP))

Binding Specificity: AA 130-136

Reactivity: Human, Mouse, Rat, Rabbit, Sheep, Goat, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This MBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: IHC-plus™

Specificity: Recognizes residues 130-136 in human myelin basic protein (21.5kD and 18.5kD molecular forms).

Purification: Protein G purified

Immunogen: Purified human myelin basic protein.

Type of Immunogen: Purified protein

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA (10 - 20 μg/mL), IHC, IHC-Fr, IHC-P (5 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, contains no preservative.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MBP

Target: MBP (Myelin Basic Protein (MBP))

Alternative Name: Myelin Basic Protein / MBP (MBP Products)

Synonyms: C76307 antibody, Hmbpr antibody, R75289 antibody, golli-mbp antibody, mld antibody, shi antibody, Mbps antibody, cb274 antibody, fj33b11 antibody, mbp antibody, wu:fj33b11 antibody, wu:fq15b02 antibody, zgc:136630 antibody, MGC115135 antibody, myelin basic protein antibody, myelin basic protein a antibody, myelin basic protein L homeolog antibody, MBP antibody, Mbp antibody, mbpa antibody, mbp antibody, mbp.L antibody

Background: Name/Gene ID: MBP

Synonyms: MBP, Myelin A1 protein, Myelin basic protein

Gene ID: 4155

UniProt: P02686