PRKG1 antibody (AA 657-671)
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- Target See all PRKG1 Antibodies
- PRKG1 (Protein Kinase, CGMP-Dependent, Type I (PRKG1))
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Binding Specificity
- AA 657-671
- Reactivity
- Human, Mouse, Rat, Pig, Cow, Chicken, Dog, Horse, Monkey, Rabbit, Bat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PRKG1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Recognizes human PKG1 alpha at ~75kD.
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%) Pufferfish (93%) Xenopus, Zebrafish (87%) Stickleback, Medaka, Seq squirt, Mosquito, Ant, Beetle (80%).
- Purification
- Protein A purified
- Immunogen
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A 15 residue synthetic peptide DEPPPDDNSGWDIDF based on the human PKG 1alpha (residues 657-671) and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%), Pufferfish (93%), Xenopus, Zebrafish (87%), Stickleback, Medaka, Seq squirt, Mosquito, Ant, Beetle (80%).
Type of Immunogen: Synthetic peptide - KLH conjugated - Isotype
- IgG
- Top Product
- Discover our top product PRKG1 Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (10 μg/mL), WB (1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Positive control: Mouse Brain Tissue Extract, Rat Brain Tissue Extract. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.2, 50 % glycerol, 0.09 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- PRKG1 (Protein Kinase, CGMP-Dependent, Type I (PRKG1))
- Alternative Name
- PKG / PRKG1 (PRKG1 Products)
- Synonyms
- prkg1 antibody, zgc:63955 antibody, Pkgi antibody, cGk1 antibody, 1 antibody, PKG antibody, PRKG1B antibody, PRKGR1B antibody, cGK antibody, cGK 1 antibody, cGK1 antibody, cGKI antibody, cGKI-BETA antibody, cGKI-alpha antibody, CGKI antibody, cGKI-beta antibody, AW125416 antibody, Gm19690 antibody, Prkg1b antibody, Prkgr1b antibody, Prkg1beta antibody, protein kinase, cGMP-dependent, type Ia antibody, protein kinase, cGMP-dependent, type I antibody, protein kinase, cGMP-dependent, type 1 antibody, prkg1a antibody, PRKG1 antibody, prkg1 antibody, Prkg1 antibody
- Background
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Name/Gene ID: PRKG1
Subfamily: CGMP
Family: Protein Kinase
Synonyms: PRKG1, CGKI, CGMP kinase I, CGMP protein kinase type I, CGK-I, CGK1, PKGI, PKG I, PRKGR1B, PRKG1B, PRKGR1A, PGK, PKG, CGK, CGK 1, CGKI-alpha, CGKI-BETA - Gene ID
- 5592
- UniProt
- Q13976
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