ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Cross-Reactivity
Chicken, Human, Mouse
Characteristics
ENO2, Enolase 2, (gamma, neuronal), 2-Phospho-D-Glycerate Hydrolase,Enolases have been characterized as highly conserved cytoplasmic glycolytic enzymes that may be involved in differentiation. Three isoenzymes have been identified, Enolase, Enolase and Enolase. Enolase expression has been detected on most tissues, whereas Enolase is expressed predominantly in muscle tissue and Enolase is detected only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway.
Purification
Protein A/G Chromatography
Immunogen
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the human NSE protein and mouse myeloma Ag8563 cells. Sequence common in human, mouse and chicken.
Antibody can be used for Western blotting (1-2 μg/mL) and immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections (1-5 μg/mL). Optimal concentration should be evaluated by serial dilutions.
Restrictions
For Research Use only
Buffer
Provided as solution in phosphate buffered saline with 0.08 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles