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Myogenin antibody (AA 30-224)

MYOG Reactivity: Human WB, ELISA, IF, IP, IHC (p), GS Host: Mouse Monoclonal F12B unconjugated
Catalog No. ABIN306849
  • Target See all Myogenin (MYOG) Antibodies
    Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))
    Binding Specificity
    • 29
    • 15
    • 15
    • 12
    • 12
    • 10
    • 9
    • 8
    • 8
    • 7
    • 7
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 30-224
    Reactivity
    • 113
    • 85
    • 58
    • 40
    • 28
    • 14
    • 13
    • 12
    Human
    Host
    • 73
    • 58
    • 1
    Mouse
    Clonality
    • 73
    • 59
    Monoclonal
    Conjugate
    • 60
    • 10
    • 10
    • 9
    • 7
    • 7
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Myogenin antibody is un-conjugated
    Application
    • 96
    • 73
    • 32
    • 30
    • 30
    • 19
    • 13
    • 12
    • 11
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Gel Shift (GS)
    Cross-Reactivity
    Human, Mouse, Rat
    Characteristics
    Myogenin, Myogenic factor 4, Myf-4,Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.
    Purification
    Protein A/G Chromatography
    Immunogen
    Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein containing rat myogenin amino acids 30-224 and mouse myeloma NS1 cells.
    Clone
    F12B
    Isotype
    IgG1
    Top Product
    Discover our top product MYOG Primary Antibody
  • Application Notes
    This antibody can be used for ELISA, gel supershift assays, immunofluorescence, immunoprecipitation of protein-DNA complexes (2 μg/mg), Western blotting (1 μg/mL) and is the antibody of choise for immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections (1-2 μg/mL). Optimal concentration should be evaluated by serial dilutions.
    Restrictions
    For Research Use only
  • Buffer
    Provided as solution in phosphate buffered saline with 0.08 % sodium azide,X1415M, clone F12B recognizes a protein of 34 kDa, identified as myogenin. Its epitope localizes between aa 158-178 of myogenin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
  • Target
    Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))
    Alternative Name
    Myogenin (MYOG Products)
    Synonyms
    cb553 antibody, zgc:100763 antibody, LOC446037 antibody, myf4 antibody, Xmyog antibody, myogenin antibody, LOC100136471 antibody, LOC100224062 antibody, LOC100303673 antibody, MYF4 antibody, bHLHc3 antibody, myo antibody, myf-4 antibody, myogenin antibody, myogenin (myogenic factor 4) antibody, MYOG antibody, myog antibody, LOC100303673 antibody, Myog antibody
    Pathways
    Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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