Tenascin is an extracellular matrix glyoprotein which is found in numerous types of tissues, but most predominately in the nervous system. It has been shown to be upregulated at the edge of healing wounds and in the stroma of many tumors. Three types of tenascin have been identified, tenascin-C, which was the first to be identified, tenascin-R, which is found almost exclusively in the central nervous system, and tenascin-X, a mammalian tenascin found in some connective tissue and around blood vessels.
Purification
Protein A/G Chromatography
Immunogen
Hybridoma produced by the fusion of splenocytes from mice immunized with protein preparation from a homogenate of a human mammary tumor and mouse myeloma cells.
Detects human tenascin protein by Western blot at 1-5 μg/mL. Can also be used for immunohistochemistry on frozen and paraffin-embedded tissue sections and for immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.
Restrictions
For Research Use only
Buffer
Provided as solution in phosphate buffered saline with 0.08 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles