PPP4R1 antibody (AA 1-205)

Catalog No. ABIN306662

Product Details anti-PPP4R1 Antibody

Target: PPP4R1 (Protein Phosphatase 4, Regulatory Subunit 1 (PPP4R1))

Binding Specificity: AA 1-205

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PPP4R1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity (Details): Human, mouse, rat

Characteristics: Serine/Threonine-Protein Phosphatase 4 Regulatory Subunit 1, Protein Phosphatase X/A'2, PP4R1,Clone 4A4 binds to all known Isotypes of p63. It is known as an excellent marker of basal cells in squamous epithelia and transitional cell epithelia (localisation of the antigen in cell nuclei). It is well suited for the detection of squamous cell carcinoma. In prostate tissue 80 % of basal cells in benign tissue react positive, whereas p63 disappears in prostate carcinoma. The p63 gene is a homologue of the tumour suppressor gene p53. It is highly expressed in nuclei of basal cells, the progenitor cells of many epithelial tissues. It shows up remarkable structural similarities to p53. By alternative splicing at least 6 different isoforms are synthesised, which are different in the C-terminus (alpha, beta, gamma) and in the N-terminus (TA and DN). In mice loss of the p63 gene leads to inability to form basal cells and severe organic deficits. Similar organic failures occur in humans with autosomal dominant EEC-Syndrome, which obviously is related to mutation of the p63 gene. p63 (6 Isotypes).

Immunogen: Recombinant p63 Protein (AA 1-205) N-terminal part of deltaNp63

Isotype: IgG

Application Details

Application Notes: Pre-treatment of formaldehyde-fixed tissue with Tris-buffered EDTA solution pH 9 or Unmasking Fluid G 40 min at 100 °C,IHC(P),60 min at RT,(liquid conc.) 1:25-1:50

Restrictions: For Research Use only

Handling

Buffer: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide. The volume is sufficient for 250-500 immunohistochemical tests (100 μL working solution / test). Use appropriate antibody diluent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for PPP4R1

Target: PPP4R1 (Protein Phosphatase 4, Regulatory Subunit 1 (PPP4R1))

Alternative Name: Protein Phosphatase 4 R1 (X/A'2) (PPP4R1 Products)

Synonyms: MGC55603 antibody, zgc:55603 antibody, ppp4r1 antibody, MGC81597 antibody, MGC89421 antibody, PPP4R1 antibody, MEG1 antibody, PP4(Rmeg) antibody, PP4R1 antibody, 3110001J10Rik antibody, Pp4r1 antibody, protein phosphatase 4 regulatory subunit 1 antibody, protein phosphatase 4, regulatory subunit 1 antibody, protein phosphatase 4 regulatory subunit 1 S homeolog antibody, PPP4R1 antibody, ppp4r1 antibody, ppp4r1.S antibody, Ppp4r1 antibody