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Claudin 12 antibody (N-Term)

CLDN12 Reactivity: Human, Mouse WB, IHC, IHC (p), IF Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN305786
  • Target See all Claudin 12 (CLDN12) Antibodies
    Claudin 12 (CLDN12)
    Binding Specificity
    • 21
    • 8
    • 7
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    • 3
    • 1
    N-Term
    Reactivity
    • 31
    • 19
    • 16
    Human, Mouse
    Host
    • 44
    • 3
    Rabbit
    Clonality
    • 45
    • 2
    Polyclonal
    Conjugate
    • 12
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Claudin 12 antibody is un-conjugated
    Application
    • 33
    • 28
    • 23
    • 13
    • 13
    • 3
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
    Brand
    IHC-plus™
    Specificity
    Recognizes mouse Claudin 12 protein at ~27kD in Western blots. Cross-reactivity has been confirmed on mouse lung, colon and kidney homogenates. Recognizes human protein in IHC-P.
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic peptide derived from the N-terminal region of mouse and human Claudin 12.

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG
    Top Product
    Discover our top product CLDN12 Primary Antibody
  • Application Notes
    Approved: IF (5 μg/mL), IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunofluorescence: 5 μg/mL, performed using ethanol-fixed mouse colon. Positive control: Mouse lung, colon, and kidney homogenates.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS,  pH 7.4, 0.1 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Aliquot to Avoid freeze/thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Target
    Claudin 12 (CLDN12)
    Alternative Name
    CLDN12 / Claudin 12 (CLDN12 Products)
    Synonyms
    CLDN12 antibody, Claudin-12 antibody, DKFZp469F1620 antibody, zgc:86588 antibody, wu:fc27c11 antibody, wu:fc37h01 antibody, claudin-12 antibody, RGD1561053 antibody, claudin 12 antibody, claudin 12 S homeolog antibody, CLDN12 antibody, cldn12 antibody, Cldn12 antibody, cldn12.S antibody, CpipJ_CPIJ011954 antibody
    Background
    Name/Gene ID: CLDN12
    Family: Claudin

    Synonyms: CLDN12, Claudin 12, Claudin-12
    Gene ID
    9069
    UniProt
    P56749
    Pathways
    Cell-Cell Junction Organization
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