Claudin 12 antibody (N-Term)

Catalog No. ABIN305786

Product Details anti-Claudin 12 Antibody

Target: Claudin 12 (CLDN12)

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Claudin 12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Brand: IHC-plus™

Specificity: Recognizes mouse Claudin 12 protein at ~27kD in Western blots. Cross-reactivity has been confirmed on mouse lung, colon and kidney homogenates. Recognizes human protein in IHC-P.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the N-terminal region of mouse and human Claudin 12.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IF (5 μg/mL), IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunofluorescence: 5 μg/mL, performed using ethanol-fixed mouse colon. Positive control: Mouse lung, colon, and kidney homogenates.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Claudin 12

Target: Claudin 12 (CLDN12)

Alternative Name: CLDN12 / Claudin 12 (CLDN12 Products)

Synonyms: CLDN12 antibody, Claudin-12 antibody, DKFZp469F1620 antibody, zgc:86588 antibody, wu:fc27c11 antibody, wu:fc37h01 antibody, claudin-12 antibody, RGD1561053 antibody, claudin 12 antibody, claudin 12 S homeolog antibody, CLDN12 antibody, cldn12 antibody, Cldn12 antibody, cldn12.S antibody, CpipJ_CPIJ011954 antibody

Background: Name/Gene ID: CLDN12
Family: Claudin

Synonyms: CLDN12, Claudin 12, Claudin-12

Gene ID: 9069

UniProt: P56749

Pathways: Cell-Cell Junction Organization