Elastin antibody

Catalog No. ABIN305753

Product Details anti-Elastin Antibody

Target: Elastin (ELN)

Reactivity: Human, Mouse, Cow, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Elastin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Brand: IHC-plus™

Specificity: Recognizes chicken Elastin.

Purification: Protein A purified

Immunogen: Tropoelastin from 17-day old lathyric chick embryo aortae, extracted and purified according to the method of Rich and Foster, Meth. Enzymol. 82:665-673(1982).

Clone: 10B8

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IF, IHC, IHC-P (5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Chicken

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Elastin

Target: Elastin (ELN)

Alternative Name: ELN / Elastin (ELN Products)

Synonyms: SVAS antibody, WBS antibody, WS antibody, AI385707 antibody, AI480567 antibody, E030024M20Rik antibody, RATTREL11 antibody, TREL11 antibody, Trela antibody, Trela26 antibody, ELN antibody, elastin antibody, ELN antibody, Eln antibody, LOC574132 antibody, Tcur_2481 antibody, Kfla_2289 antibody, LOC100620140 antibody

Background: Name/Gene ID: ELN

Synonyms: ELN, SVAS, WBS, Elastin, Tropoelastin, WS

Gene ID: 2006

UniProt: P15502

Pathways: Regulation of Actin Filament Polymerization