SAA1 antibody
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- Target See all SAA1 Antibodies
- SAA1 (Serum Amyloid A1 (SAA1))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This SAA1 antibody is un-conjugated
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Recognizes natural and recombinant human SAA. Does not show any cross-reactivity with other human cytokines or growth factors tested such as IL-1b, IL-8, MCAF, TGF-b and EGF.
- Purification
- Protein G purified
- Immunogen
- Highly purified recombinant human SAA, 12kD.
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product SAA1 Primary Antibody
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- Application Notes
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Approved: ELISA (0.3 μg/mL), IHC, IHC-P (5 μg/mL), SwELISA, WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA (capture coating): 0.3 μg/mL Detects 1.1 ng/mL of SAA in serum/plasma or 0.6 ng/mL of SAA in medium with an assay range of 0 to 80 ng/mL. Suggested substrate: TMB. Western Blot: 0.1-0.5 μg allows visualization of 100 ng/lane of recombinant human SAA. Used in sandwich ELISA as the capture antibody with LS-B2208 as the detection antibody. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.2
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- SAA1 (Serum Amyloid A1 (SAA1))
- Alternative Name
- SAA1 / SAA / Serum Amyloid A (SAA1 Products)
- Background
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Name/Gene ID: SAA1
Synonyms: SAA1, Serum amyloid A protein, Serum Amyloid A, Serum amyloid A-1 protein, TP53I4, SAA, Serum amyloid A1, PIG4 - Gene ID
- 6288
- UniProt
- P02735
- Pathways
- Toll-Like Receptors Cascades
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