H-2Dd antibody

Catalog No. ABIN3044603

Product Details anti-H-2Dd Antibody

Target: H-2Dd (MHC Class I H-2Dd)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This H-2Dd antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Characteristics: The 34-2-12 monoclonal antibody reacts with the mouse alpha3 domain of H-2Dd MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dd haplotype.

Immunogen: C57BL/6 x DBA/2)F1 mouse splenocytes

Clone: 34-2-12

Isotype: IgG2a

Application Details

Application Notes: It is recommended for use in flow cytometry. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent iless or equal to 1 µg per 10^6 cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Comment:

Purified, unconjugated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 0,09% Sodium azide, pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for H-2Dd

Target: H-2Dd (MHC Class I H-2Dd)

Alternative Name: H-2Dd (H-2Dd Products)

Background: The 34-2-12 antibody (also known as 34-2-12S) recognizes the alpha3 domain of the H-2D^[d]. The binding of the antibody to its epitope is independent of the alpha1 and alpha2 domains and beta2 microglobulin. It cross-reacts with cells of the C3H.LG/Ckc strain. Reactivity with other haplotypes (eg, b, f, k, p, q, r, s) has not been observed. Soluble mAb 34-2-12 blocks binding of the Ly-49A-expressing T lymphoma EL4 to immobilized H-2D[d]. However, further studies utilizing this mAb indicate that the alpha3 domain is not involved in the interaction between Ly-49A, or Ly-49G2, and H-2D[d].