SFRP4 antibody (AA 22-303)

Catalog No. ABIN3044543

Product Details anti-SFRP4 Antibody

Target: SFRP4 (Secreted Frizzled-Related Protein 4 (SFRP4))

Binding Specificity: AA 22-303

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SFRP4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Secreted frizzled-related protein 4(SFRP4) detection. Tested with WB, IHC-P, ELISA in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Secreted frizzled-related protein 4(SFRP4) detection. Tested with WB, IHC-P, ELISA in Human.
Gene Name: secreted frizzled-related protein 4
Protein Name: Secreted frizzled-related protein 4

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human SFRP4 recombinant protein (Position: A22-K303). Human SFRP4 shares 96.8% and 96.1% amino acid (aa) sequence identity with mouse and rat SFRP4, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Human

Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for SFRP4

Target: SFRP4 (Secreted Frizzled-Related Protein 4 (SFRP4))

Alternative Name: SFRP4 (SFRP4 Products)

Synonyms: SFRP4 antibody, FRP-4 antibody, FRPHE antibody, sFRP-4 antibody, secreted frizzled related protein 4 antibody, secreted frizzled-related protein 4 antibody, SFRP4 antibody, Sfrp4 antibody

Background: Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. It is mapped to 7p14.1. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. And SFRP4 is a hub gene in a Type 2 Diabetes-associated gene coexpression module in human islets, and reduces glucose-induced insulin secretion through decreased β-cell exocytosis.

Synonyms: Frizzled protein antibody|Frizzled protein human endometrium antibody|FRP 4 antibody|FRP4 antibody|Fr pH E antibody|human endometrium antibody|Secreted frizzled-related protein 4 antibody|sFRP-4 antibody|Sfrp4 antibody|SFRP4_HUMAN antibody

Gene ID: 6424

Pathways: WNT Signaling