Nestin antibody (N-Term)

Catalog No. ABIN3044529

Product Details anti-Nestin Antibody

Target: Nestin (NES)

Binding Specificity: AA 252-290, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Protein Mdm4(MDM4) detection. Tested with WB, IHC-P in Human,Rat.

Sequence: ERLRATEKFQ LAVEALEQEK QGLQSQIAQV LEGRQQLAH

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Protein Mdm4(MDM4) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: nestin
Protein Name: Nestin

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Nestin (252-290aa ERLRATEKFQLAVEALEQEKQGLQSQIAQVLEGRQQLAH), different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-Nestin antibody (ABIN3044529)

Li, Xie, Yu, He: "Increased Efficacy of Antivenom Combined with Hyperbaric Oxygen on Deinagkistrodon acutus Envenomation in Adult Rats." in: Chinese medical journal, Vol. 131, Issue 3, pp. 323-329, (2018) (PubMed).

Luo, Deng, Yi, Zhou, She, Liu: "Buyang Huanwu Decoction Ameliorates Poststroke Depression via Promoting Neurotrophic Pathway Mediated Neuroprotection and Neurogenesis." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 4072658, (2017) (PubMed).

Zhu, Liu, Wang, Nie, He, Zhang, Liu, Su: "Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats." in: Molecular medicine reports, Vol. 12, Issue 4, pp. 5691-700, (2016) (PubMed).

Yang, Li, Li, Feng, Liu, Han, Zhang, Qin, Zhang, Nie, Jin: "Effect of Corilagin on the Proliferation and NF-κB in U251 Glioblastoma Cells and U251 Glioblastoma Stem-Like Cells." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 1418309, (2016) (PubMed).

Zeng, Zhao, Ouyang, Zhao, Zhang, Chen, Yu, Lei: "Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium." in: Oncology letters, Vol. 12, Issue 2, pp. 815-824, (2016) (PubMed).

Li, Lu, Wang: "Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cells." in: Neural regeneration research, Vol. 9, Issue 22, pp. 2002-7, (2015) (PubMed).

Lu, Li, Lu: "Distribution and localization of fibroblast growth factor-8 in rat brain and nerve cells during neural stem/progenitor cell differentiation." in: Neural regeneration research, Vol. 7, Issue 19, pp. 1455-62, (2015) (PubMed).

Chen, Zhang, Liu, He, Zhou, Shao, Sun, Cao, Gong, Jiang: "A fibrin matrix promotes the differentiation of EMSCs isolated from nasal respiratory mucosa to myelinating phenotypical Schwann-like cells." in: Molecules and cells, Vol. 38, Issue 3, pp. 221-8, (2015) (PubMed).

Liu, Guo, Zhang, Li, Feng, Yu, Kong, Zhao, Jin: "Effect of siRNA-Livin on drug resistance to chemotherapy in glioma U251 cells and CD133+ stem cells." in: Experimental and therapeutic medicine, Vol. 10, Issue 4, pp. 1317-1323, (2015) (PubMed).

Wei, Yang, Hou, Liu, Gao, Zhang, Wang, Mao, Gao, Chen, Hong: "Insulin-producing cells derived from human embryonic stem cells: comparison of definitive endoderm- and nestin-positive progenitor-based differentiation strategies." in: PLoS ONE, Vol. 8, Issue 8, pp. e72513, (2014) (PubMed).

Xiong, Zhou, Wang, Xue, Wang, Sun, Ge: "Impaired spatial learning and memory after sevoflurane-nitrous oxide anesthesia in aged rats is associated with down-regulated cAMP/CREB signaling." in: PLoS ONE, Vol. 8, Issue 11, pp. e79408, (2014) (PubMed).

Zhang, Pei, Ru, Li, Liu: "Bone morphogenetic protein 7 upregulates the expression of nestin and glial fibrillary acidic protein in rats with cerebral ischemia-reperfusion injury." in: Biomedical reports, Vol. 1, Issue 6, pp. 895-900, (2014) (PubMed).

Xu, Sun, Wu, Xi: "Investigation of characteristics of feather follicle stem cells and their regeneration potential." in: Journal of stem cells & regenerative medicine, Vol. 7, Issue 2, pp. 69-74, (2014) (PubMed).

Fu, Lv, Zhuang, Chen, Lv, Li, Wang: "An effective inducer of dopaminergic neuron-like differentiation." in: Neural regeneration research, Vol. 8, Issue 5, pp. 427-34, (2014) (PubMed).

Yang, Zhu, Zhang, Gao, Cao: "Ipsilateral versus bilateral limb-training in promoting the proliferation and differentiation of endogenous neural stem cells following cerebral infarction in rats." in: Neural regeneration research, Vol. 7, Issue 34, pp. 2698-704, (2014) (PubMed).

Hu, Deng, Liu, Chen, Zhang, Sun, Dong, Liu, Ge: "Characterization and retinal neuron differentiation of WERI-Rb1 cancer stem cells." in: Molecular vision, Vol. 18, pp. 2388-97, (2013) (PubMed).

Bin, Ma, Xu, Shi: "Embryonic hepatocyte transplantation for hepatic cirrhosis: efficacy and mechanism of action." in: World journal of gastroenterology, Vol. 18, Issue 4, pp. 309-22, (2012) (PubMed).

Yang, Zhou, Wang, Wang: "Differentiation of human bone marrow-derived mesenchymal stem cells into neural-like cells by co-culture with retinal pigmented epithelial cells." in: International journal of ophthalmology, Vol. 3, Issue 1, pp. 23-7, (2012) (PubMed).

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: NES (NES Products)

Synonyms: paranemin antibody, NES antibody, AA166324 antibody, C78523 antibody, ESTM46 antibody, nestin antibody, nestin S homeolog antibody, NES antibody, nes.S antibody, Nes antibody, nes antibody

Background: Nestin is a type VI intermediate filament (IF) protein that in humans is encoded by the NES gene. This gene is mapped to 1q23.1. Distribution and expression of nestin in mitotically active cells suggests it plays a role in regulation of the assembly and disassembly of intermediate filaments, which, together with other structural proteins, participate in remodeling of the cell. The role of nestin in dynamic cells, particularly structural organization of the cell, appears strictly regulated by phosphorylation, especially its integration into heterogeneous intermediate filaments together with vimentin or α-internexin. Furthermore, nestin expression has been extensively used as a marker for central nervous system (CNS) progenitor cells in different contexts, based on observations indicating a correlation between nestin expression and this cell type in vivo.

Synonyms: ESTM 46 antibody|FLJ 21841 antibody|FLJ21841 antibody|Intermediate filament protein antibody|Nbla00170 antibody|nes antibody| NEST_HUMAN antibody|Nestin antibody

Gene ID: 10763

UniProt: P48681