Fatty Acid Synthase antibody (AA 1-226)

Catalog No. ABIN3044520

Product Details anti-Fatty Acid Synthase Antibody

Target: Fatty Acid Synthase (FASN)

Binding Specificity: AA 1-226

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fatty Acid Synthase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Fatty acid synthase(FASN) detection. Tested with WB, IHC-P in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Fatty acid synthase(FASN) detection. Tested with WB, IHC-P in Human.
Gene Name: fatty acid synthase
Protein Name: Fatty acid synthase

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human FASN recombinant protein (Position: M1-E226). Human FASN shares 90.3% and 90.7% amino acid (aa) sequence identity with mouse and rat FASN, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-Fatty Acid Synthase antibody (ABIN3044520)

Lu, Ma, Cai: "Increased HAGLR expression promotes non-small cell lung cancer proliferation and invasion via enhanced de novo lipogenesis." in: Tumour biology, Vol. 39, Issue 4, pp. 1010428317697570, (2017) (PubMed).

Target Details for Fatty Acid Synthase

Target: Fatty Acid Synthase (FASN)

Alternative Name: FASN (FASN Products)

Synonyms: FAS antibody, OA-519 antibody, SDR27X1 antibody, FASN antibody, fj34h12 antibody, wu:fj34h12 antibody, Cgl0836 antibody, A630082H08Rik antibody, BcDNA:GH07626 antibody, BcDNA:gh07626 antibody, DM_7295848 antibody, Dmel\\CG3523 antibody, Fatty acid synthase antibody, anon-EST:Posey235 antibody, anon-EST:Posey43 antibody, anon-WO0138581.1 antibody, dFAS antibody, fas antibody, fatty acid synthase antibody, fatty acid synthase Fas antibody, type I polyketide synthase antibody, Fatty acid synthase Fas antibody, Fatty Acid SyNthase antibody, fatty-acid synthase antibody, fatty-acid synthase II antibody, fatty-acid synthase fas antibody, Fatty acid synthase 1 antibody, FASN antibody, fas antibody, Fasn antibody, BAD_RS01360 antibody, fasn antibody, fasn-1 antibody, NCgl0802 antibody, fasB antibody, MT2600 antibody, TBMG_01448 antibody, FASN1 antibody

Background: Fatty acid synthase (FAS) is an enzyme that in humans is encoded by the FASN gene. It is mapped to 17q25. The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Synonyms: [Acyl-carrier-protein] S acetyltransferase antibody|[Acyl-carrier-protein] S malonyltransferase antibody|3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody|3-oxoacyl-[acyl-carrier-protein] reductase antibody|3-oxoacyl-[acyl-carrier-protein] synthase antibody|Enoyl-[acyl-carrier-protein] reductase antibody|FAS antibody|FAS_HUMAN antibody|FASN antibody|Fatty acid synthase antibody| MGC14367 antibody|MGC15706 antibody|OA 519 antibody|Oleoyl-[acyl-carrier-protein] hydrolase antibody|SDR27X1 antibody|Short chain dehydrogenase/reductase family 27X member 1 antibody

Gene ID: 2194

UniProt: P49327

Pathways: AMPK Signaling