DIABLO antibody (AA 56-239)

Catalog No. ABIN3044515

Product Details anti-DIABLO Antibody

Target: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Binding Specificity: AA 56-239

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIABLO antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Diablo homolog, mitochondrial(DIABLO) detection. Tested with WB, IHC-P, ELISA in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Diablo homolog, mitochondrial(DIABLO) detection. Tested with WB, IHC-P, ELISA in Human,Mouse,Rat.
Gene Name: diablo, IAP-binding mitochondrial protein
Protein Name: Diablo homolog, mitochondrial

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Smac/Diablo recombinant protein (Position: A56-D239). Human Smac/Diablo shares 88.6% amino acid (aa) sequence identity with mouse Smac/Diablo.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Human

Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-DIABLO antibody (ABIN3044515)

You, Li, Jin, Zheng, Chen, Yang: "Genistein protects against Aβ25-35 induced apoptosis of PC12 cells through JNK signaling and modulation of Bcl-2 family messengers." in: BMC neuroscience, Vol. 18, Issue 1, pp. 12, (2017) (PubMed).

Target Details for DIABLO

Target: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Alternative Name: DIABLO (DIABLO Products)

Synonyms: smac antibody, smac3 antibody, diablo-s antibody, 0610041G12Rik antibody, 1700006L01Rik antibody, AU040403 antibody, Smac antibody, DFNA64 antibody, SMAC antibody, DBO antibody, XSmac antibody, diablo, IAP-binding mitochondrial protein antibody, diablo IAP-binding mitochondrial protein antibody, diablo, IAP-binding mitochondrial protein L homeolog antibody, diablo antibody, Diablo antibody, DIABLO antibody, diablo.L antibody

Background: Diablo homolog (DIABLO), also referred to as second mitochondria-derived activator of caspases or SMAC, is a mitochondrial protein that in humans is encoded by the DIABLO (direct IAP binding protein with low pI) gene on chromosome 12. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms.

Synonyms: 0610041G12Rik antibody|DBLOH_HUMAN antibody|DBOH antibody|diablo antibody|Diablo homolog (Drosophila) antibody|Diablo homolog antibody|Diablo homolog mitochondrial antibody|DIABLO S antibody|Direct IAP binding protein with low pI antibody|Direct IAP-binding protein with low pI antibody|FLJ10537 antibody|FLJ25049 antibody|mitochondrial antibody|Mitochondrial Smac protein antibody|Second mitochondria derived activator of caspase antibody|Second mitochondria-derived activator of caspase antibody|SMAC 3 antibody|Smac antibody|Smac protein antibody|SMAC3 antibody

Gene ID: 56616

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity