SCG3 antibody (C-Term)

Catalog No. ABIN3044496

Product Details anti-SCG3 Antibody

Target: SCG3 (Secretogranin III (SCG3))

Binding Specificity: AA 438-450, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCG3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for Secretogranin-3(SCG3) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.

Sequence: DFINKQADAY VEK

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Secretogranin-3(SCG3) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: secretogranin III
Protein Name: Secretogranin-3

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Secretogranin 3(438-450aa DFINKQADAYVEK), different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for SCG3

Target: SCG3 (Secretogranin III (SCG3))

Alternative Name: SCG3 (SCG3 Products)

Synonyms: SgIII antibody, scg3-A antibody, zgc:73236 antibody, sgiii antibody, DKFZp459J1919 antibody, SGIII antibody, 1B1075 antibody, AI385542 antibody, Chgd antibody, secretogranin III L homeolog antibody, secretogranin III antibody, scg3.L antibody, scg3 antibody, SCG3 antibody, Scg3 antibody

Background: SCG3(secretogranin III) is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. Genetic variations in the SCG3 gene may influence the risk of obesity through possible regulation of hypothalamic neuropeptide secretion. SCG3 was the only gene within a haplotype block that contained rs3764220. SCG3 mRNA and immunoreactivity were detected in the paraventricular nucleus, lateral hypothalamic area, and arcuate nucleus, and the protein coexisted with orexin, melanin-concentrating hormone, neuropeptide Y, and proopiomelanocortin. SCG3 formed a granule-like structure together with these neuropeptides.

Synonyms: 1B1075 antibody|AI385542 antibody|Chgd antibody|SCG3 antibody|SCG3_HUMAN antibody|Secretogranin III antibody|Secretogranin-3 antibody|SGCG antibody|SgIII antibody