PROC antibody (C-Term)

Catalog No. ABIN3044486

Product Details anti-PROC Antibody

Target: PROC (Vitamin K-dependent protein C (PROC))

Binding Specificity: AA 446-461, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PROC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Vitamin K-dependent protein C(PROC) detection. Tested with WB, IHC-P in Human.

Sequence: HGHIRDKEAP QKSWAP

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Vitamin K-dependent protein C(PROC) detection. Tested with WB, IHC-P in Human.
Gene Name: protein C(inactivator of coagulation factors Va and VIIIa)
Protein Name: Vitamin K-dependent protein C

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Protein C(446-461aa HGHIRDKEAPQKSWAP).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-PROC antibody (ABIN3044486)

Liu, An, Liu, Li, Tang, Chen: "Mouse lung slices: An ex vivo model for the evaluation of antiviral and anti-inflammatory agents against influenza viruses." in: Antiviral research, Vol. 120, pp. 101-11, (2015) (PubMed).

Li, Wang, Zhao, Yang, Li, Zhao, Yu, Wang, Meng, Liu, Zhu, Gao, Jia, Yang: "Nuclear factor of activated T cells 5 maintained by Hotair suppression of miR-568 upregulates S100 calcium binding protein A4 to promote breast cancer metastasis." in: Breast cancer research : BCR, Vol. 16, Issue 5, pp. 454, (2015) (PubMed).

Ban, Fan, Huang, Li, Chen, Zhang, Xu: "Mono-(2-ethylhexyl) phthalate induces injury in human umbilical vein endothelial cells." in: PLoS ONE, Vol. 9, Issue 5, pp. e97607, (2014) (PubMed).

Tang, Li, Yu, Gao, Liu, Chen, Xing, Liu, Yao: "Quercetin prevents ethanol-induced iron overload by regulating hepcidin through the BMP6/SMAD4 signaling pathway." in: The Journal of nutritional biochemistry, Vol. 25, Issue 6, pp. 675-82, (2014) (PubMed).

Zhang, Zhang, Goldstein, Li, Ge, He, Ruiz: "The effect of c-fos on acute myocardial infarction and the significance of metoprolol intervention in a rat model." in: Cell biochemistry and biophysics, Vol. 65, Issue 2, pp. 249-55, (2013) (PubMed).

Aksu, Baykara, Kiray, Gurpinar, Sisman, Ekerbicer, Tas, Gokdemir-Yazar, Uysal: "Serum IGF-1 levels correlate negatively to liver damage in diabetic rats." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, Vol. 88, Issue 3-4, pp. 194-201, (2013) (PubMed).

Chen, Liu, Yang, Leng: "Characterization of bmp15 and its regulation by human chorionic gonadotropin in the follicle of gibel carp (Carassius auratus gibelio)." in: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, Vol. 163, Issue 1, pp. 121-8, (2012) (PubMed).

Yang, Gu, Zheng, Li, Lian, Miao, Jiang, Wei: "RPB5-mediating protein is required for the proliferation of hepatocellular carcinoma cells." in: The Journal of biological chemistry, Vol. 286, Issue 13, pp. 11865-74, (2011) (PubMed).

Bao, Liu, Qin, Xu, Hei, Jaber, Chen: "An immunohistochemical study of S-100 protein in the intestinal tract of Chinese soft-shelled turtle, Pelodiscus sinensis." in: Research in veterinary science, Vol. 91, Issue 3, pp. e16-24, (2011) (PubMed).

Zhang, He, Goldstein, Ge, Wang, Ruiz: "The expression and significance of proto-oncogene c-fos in viral myocarditis." in: Virology journal, Vol. 7, pp. 285, (2010) (PubMed).

Target Details for PROC

Target: PROC (Vitamin K-dependent protein C (PROC))

Alternative Name: PROC (PROC Products)

Synonyms: proc antibody, si:ch1073-188c16.3 antibody, zgc:63987 antibody, PROC antibody, APC antibody, PC antibody, PROC1 antibody, THPH3 antibody, THPH4 antibody, proc1 antibody, MGC64425 antibody, PA antibody, protein C, inactivator of coagulation factors Va and VIIIa antibody, protein C (inactivator of coagulation factors Va and VIIIa), a antibody, protein C (inactivator of coagulation factors Va and VIIIa) antibody, protein C, inactivator of coagulation factors Va and VIIIa S homeolog antibody, vitamin K-dependent protein C antibody, prosaposin antibody, protein C antibody, proline rich protein HaeIII subfamily 1 antibody, PROC antibody, proca antibody, proc.S antibody, proc antibody, CpipJ_CPIJ000393 antibody, CpipJ_CPIJ002754 antibody, CpipJ_CPIJ003717 antibody, CpipJ_CPIJ003718 antibody, CpipJ_CPIJ014440 antibody, CpipJ_CPIJ018032 antibody, CpipJ_CPIJ018737 antibody, PSAP antibody, Proc antibody, PRH1 antibody

Target Type: Viral Protein

Background: Protein C(PROC), also called PC, is a zymogenic(inactive) protein, the activated form of which plays an important role in regulating blood clotting, inflammation, cell death and maintaining the permeability of blood vessel walls in humans and other animals. The PROC gene is mapped on 2q14.3. The PROC gene contains 8 exons and spans about 11 kb by Foster et al. The conversion of protein C to a protease with anticoagulant function by thrombin requires as a cofactor thrombomodulin, an endothelial cell membrane protein. Riewald et al. demonstrated that activated protein C uses the endothelial cell protein C receptor as a coreceptor for cleavage of protease-activated receptor-1 on endothelial cells. Faust et al. demonstrated that the endothelial pathways required for protein C activation are impaired in severe meningococcal sepsis. They stated that improvement in the outcome of children with meningococcal sepsis who were treated with unactivated protein C concentrates had been described in case reports and in 1 uncontrolled series.

Synonyms: Activation peptide antibody|Anticoagulant protein C antibody|APC antibody|Autoprothrombin IIA antibody|Blood coagulation factor XIV antibody|EC 3.4.21.69 antibody|PC antibody|proC antibody|PROC_HUMAN antibody|PROC1 antibody|Protein C(inactivator of coagulation factors Va and VIIIa) antibody|THPH3 antibody|THPH4 antibody|Vitamin K dependent protein C antibody|Vitamin K dependent protein C precursor antibody

UniProt: P04070