ONECUT1 antibody (N-Term)

Catalog No. ABIN3044467

Product Details anti-ONECUT1 Antibody

Target: ONECUT1 (One Cut Homeobox 1 (ONECUT1))

Binding Specificity: AA 187-203, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ONECUT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for Hepatocyte nuclear factor 6(ONECUT1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.

Sequence: LGSIHNSQQG LPHYAHP

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Hepatocyte nuclear factor 6(ONECUT1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: one cut homeobox 1
Protein Name: Hepatocyte nuclear factor 6

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human HNF6(187-203aa LGSIHNSQQGLPHYAHP), identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ONECUT1

Target: ONECUT1 (One Cut Homeobox 1 (ONECUT1))

Alternative Name: ONECUT1 (ONECUT1 Products)

Synonyms: D9Ertd423e antibody, HNF-6 antibody, Hfh12 antibody, Hnf6 antibody, Hnf6b antibody, OC-1 antibody, Oc1 antibody, onecut antibody, onecut1 antibody, id:ibd5005 antibody, HNF6 antibody, HNF6A antibody, HNF-6a antibody, oc1 antibody, onecut1l antibody, zgc:65904 antibody, zgc:77450 antibody, one cut domain, family member 1 antibody, one cut homeobox 1 antibody, one cut domain, family member, like antibody, Onecut1 antibody, onecutl antibody, ONECUT1 antibody, onecut1 antibody

Background: One cut homeobox 1(ONECUT1), also called Hepatocyte nuclear factor 6(HNF6) is found strong expression in liver and lower expression in testis and skin. ONECUT1 gene is mapped to 15q21.3. This gene encodes a member of the Cut homeobox family of transcription factors. Expression of the encoded protein is enriched in the liver, where it stimulates transcription of liver-expressed genes, and antagonizes glucocorticoid-stimulated gene transcription. This gene may influence a variety of cellular processes including glucose metabolism, cell cycle regulation, and it may also be associated with cancer.

Synonyms: Hepatocyte nuclear factor 6 antibody|HNF 6 antibody|HNF6A antibody|One cut domain family member 1 antibody|Onecut1 antibody