HMMR antibody (C-Term)

Catalog No. ABIN3044455

Product Details anti-HMMR Antibody

Target: HMMR (Hyaluronan-Mediated Motility Receptor (RHAMM) (HMMR))

Binding Specificity: AA 706-724, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMMR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Hyaluronan mediated motility receptor(HMMR) detection. Tested with WB, IHC-P in Human.

Sequence: KEGNTNCYRA PMECQESWK

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Hyaluronan mediated motility receptor(HMMR) detection. Tested with WB, IHC-P in Human.
Gene Name: hyaluronan-mediated motility receptor(RHAMM)
Protein Name: Hyaluronan mediated motility receptor

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CD168(706-724aa KEGNTNCYRAPMECQESWK).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-HMMR antibody (ABIN3044455)

Ozbilgin, Boz, Tuğyan, Inan, Vatansever: "RHAMM Expression in the Rat Endometrium during the Estrous Cycle and following Implantation." in: Journal of reproduction & infertility, Vol. 13, Issue 3, pp. 131-7, (2013) (PubMed).

Target Details for HMMR

Target: HMMR (Hyaluronan-Mediated Motility Receptor (RHAMM) (HMMR))

Alternative Name: HMMR (HMMR Products)

Synonyms: AA386826 antibody, CD168 antibody, Rhamm antibody, RHAMM antibody, IHABP antibody, hyaluronan mediated motility receptor (RHAMM) antibody, hyaluronan-mediated motility receptor antibody, hyaluronan mediated motility receptor antibody, Hmmr antibody, HMMR antibody

Background: HMMR, Hyaluronan-mediated motility receptor, is a protein which in humans is encoded by the HMMR gene. RHAMM was originally discovered as a soluble protein that altered migratory cell behavior and bound to hyaluronan, HMMR is less well studied than the main hyaluronan(HA) receptor, CD44. In contrast to CD44 and other cell-surface receptors which contain the classical membrane spanning domain and signal sequence for secretion from the endoplasmic reticulum/Golgi complex, HMMR does not contain a membrane spanning domain nor does the mRNA transcript contain a signal sequence. HMMR normally is localized inside the cell and is only release by certain, poorly defined stimuli. The transport of HMMR to the extracellular space still is unclear but may involve transport channels or proteins, flippase activity, or exocytosis. Intracellularly, HMMR associates with microtubules and, working with BRCA1 and BARD1, plays a role in the regulation of mitosis. Extracellularly, HMMR associates with CD44, and upon binding to HA, activates intracellular signaling pathways. Variants of HMMR caused by alternative splicing have been observed, but not thoroughly studied.

Synonyms: CD168 antibody|CD168 antigen antibody|HMMR antibody|HMMR_HUMAN antibody|Hyaluronan mediated motility receptor antibody|Hyaluronan-mediated motility receptor(RHAMM) antibody|IHABP antibody|Intracellular hyaluronic acid-binding protein antibody|MGC119494 antibody|MGC119495 antibody|OTTHUMP00000196920 antibody|Receptor for hyaluronan-mediated motility antibody|RHAMM antibody

UniProt: O75330

Pathways: Glycosaminoglycan Metabolic Process