CAMKK1 antibody (N-Term)

Catalog No. ABIN3044431

This Rabbit Polyclonal antibody specifically detects CAMKK1 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CAMKK1 antibody (N-Term) (ABIN3044431)

Target: CAMKK1 (Calcium/calmodulin-Dependent Protein Kinase Kinase 1, alpha (CAMKK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAMKK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CAMKK1 Antibody

Binding Specificity: AA 10-31, N-Term

Purpose: Anti-CaMKK/CAMKK1 Antibody Picoband®

Sequence: QDPRAELVDR VAAINVAHLE EA

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CaMKK/CAMKK1 Antibody (ABIN3044431). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse CaMKK, different from the related human sequence by three amino acids, and from the related rat sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Blaeser, F., Sanders, M. J., Truong, N., Ko, S., Wu, L. J., Wozniak, D. F., Fanselow, M. S., Zhuo, M., Chatila, T. A.Long-term memory deficits in pavlovian fear conditioning in Ca(2+)/calmodulin kinase kinase alpha-deficient mice.Molec. Cell. Biol. 26: 9105-9115, 2006. 2. Tokumitsu, H., Enslen, H., Soderling, T. R.Characterization of a Ca(2+)/calmodulin-dependent protein kinase cascade: molecular cloning and expression of calcium/calmodulin-dependent protein kinase kinase.J. Biol. Chem. 270: 19320-19324, 1995.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CAMKK1

Target: CAMKK1 (Calcium/calmodulin-Dependent Protein Kinase Kinase 1, alpha (CAMKK1))

Alternative Name: CAMKK1

Background:

Synonyms: Calcium/calmodulin-dependent protein kinase kinase 1,CaM-KK 1,CaM-kinase kinase 1,CaMKK 1,2.7.11.17,CaM-kinase IV kinase,Calcium/calmodulin-dependent protein kinase kinase alpha,CaM-KK alpha,CaM-kinase kinase alpha,CaMKK alpha,Camkk1,Camkk,

Tissue Specificity: Widely expressed. Differentially expressed in various brain regions. .

Background: CAMKK1, Calcium/calmodulin-dependent protein kinase kinase 1 is an enzyme that in humans is encoded by the CAMKK1 gene. The CAMKK1 gene is mapped to chromosome 17. The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca (2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene.Camkk1binds calmodulin and activated Camk4 with a 6-fold increase in total activity and a 100-fold increase in Camk4 Ca (2+)-independent activity. Camkk1 catalyzed a 10-fold increase in the total activity of Camk1 and had no effect on Camk2. Cotransfection of COS-7 cells with Camkk1 and Camk4 resulted in a 14-fold increase in CRE-binding protein-dependent transcription compared with Camk4 alone, suggesting that Camkk1 enhances Camk4-mediated transcriptional regulation.

Sequence Similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Molecular Weight: 56 kDa

Pathways: AMPK Signaling