CD68 antibody (Middle Region)

Catalog No. ABIN3044428

Product Details anti-CD68 Antibody

Target: CD68 (CD68 Molecule (CD68))

Binding Specificity: AA 312-326, Middle Region

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD68 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for Macrosialin(CD68) detection. Tested with WB, IHC-P, IHC-F in Mouse,Rat.

Sequence: AFCITRRRQS TYQPL

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Macrosialin(CD68) detection. Tested with WB, IHC-P, IHC-F in Mouse,Rat.
Gene Name: CD68 Molecule
Protein Name: Macrosialin

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse CD68(312-326aa AFCITRRRQSTYQPL), different from the related rat sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-CD68 antibody (ABIN3044428)

Spronck, Ramachandra, Moriyama, Toczek, Han, Sadeghi, Humphrey: "Deletion of matrix metalloproteinase-12 compromises mechanical homeostasis and leads to an aged aortic phenotype in young mice." in: Journal of biomechanics, Vol. 141, pp. 111179, (2022) (PubMed).

Liu, Huang, Su, Song, Lu: "Blockage of Galectin-receptor Interactions by α-lactose Exacerbates Plasmodium berghei-induced Pulmonary Immunopathology." in: Scientific reports, Vol. 6, pp. 32024, (2018) (PubMed).

Yin, Chang, Xu: "Expressions Profiles of the Proteins Associated with Carbohydrate Metabolism in Rat Liver Regeneration." in: BioMed research international, Vol. 2017, pp. 8428926, (2018) (PubMed).

Li, Fang, Tian, Wang, Ao, Hou, Tong, Fan, Bai: "Curcumin attenuates the development of thoracic aortic aneurysm by inhibiting VEGF expression and inflammation." in: Molecular medicine reports, Vol. 16, Issue 4, pp. 4455-4462, (2018) (PubMed).

Chen, Shi, Liu, Ren, He, Chang, Yin: "Activation of corticotropin-releasing factor receptor 1 aggravates dextran sodium sulphate-induced colitis in mice by promoting M1 macrophage polarization." in: Molecular medicine reports, Vol. 17, Issue 1, pp. 234-242, (2018) (PubMed).

Pang, Zhang, Huang, Xu, Wang, Wang, Guan, Zhang, Yu, Chen, Xie: "Effects and Mechanisms of Total Flavonoids from Blumea balsamifera (L.) DC. on Skin Wound in Rats." in: International journal of molecular sciences, Vol. 18, Issue 12, (2018) (PubMed).

Hoffman, Adeli: "LDL Receptor Gene-Ablated Hamsters: A Rodent Model of Familial Hypercholesterolemia with Dominant Inheritance and Diet-Induced Coronary Atherosclerosis." in: EBioMedicine, Vol. 28, pp. 17-18, (2018) (PubMed).

Lin, Li, Cai, Huang, Huang, Guo, Yang, Yang, Lan, Zhu: "Andrographolide Ameliorates Liver Fibrosis in Mice: Involvement of TLR4/NF-κB and TGF-β1/Smad2 Signaling Pathways." in: Oxidative medicine and cellular longevity, Vol. 2018, pp. 7808656, (2018) (PubMed).

Guo, Liao, Huang, Wang, Huang, Liu: "Reversal of adipose tissue loss by probucol in mice with deficiency of both scavenger receptor class B type 1 and LDL receptor on high fat diet." in: Biochemical and biophysical research communications, (2017) (PubMed).

Wang, Liu, Xiong, Xu, Shi, Qiu, Zang, Mao, Zhuang: "Inhibition of EGF Receptor Blocks the Development and Progression of Peritoneal Fibrosis." in: Journal of the American Society of Nephrology : JASN, Vol. 27, Issue 9, pp. 2631-44, (2017) (PubMed).

Liu, Xiao, Liu, Li, Zhou, Xian, Wang, Zhang, Wang, Ho, Zhuang: "SIV Infection Impairs the Central Nervous System in Chinese Rhesus Macaques." in: Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, Vol. 11, Issue 3, pp. 592-600, (2017) (PubMed).

Yang, Zhang, Yu, Tang, Gan: "Renin-angiotensin system activation accelerates atherosclerosis in experimental renal failure by promoting endoplasmic reticulum stress-related inflammation." in: International journal of molecular medicine, Vol. 39, Issue 3, pp. 613-621, (2017) (PubMed).

Li, Lu, Sun, Zuo, Wang, Yan: "Inhibition of endoplasmic reticulum stress signaling pathway: A new mechanism of statins to suppress the development of abdominal aortic aneurysm." in: PLoS ONE, Vol. 12, Issue 4, pp. e0174821, (2017) (PubMed).

Leftin, Ben-Chetrit, Klemm, Joyce, Koutcher: "Iron imaging reveals tumor and metastasis macrophage hemosiderin deposits in breast cancer." in: PLoS ONE, Vol. 12, Issue 9, pp. e0184765, (2017) (PubMed).

Leftin, Zhao, Turkekul, de Stanchina, Manova, Koutcher: "Iron deposition is associated with differential macrophage infiltration and therapeutic response to iron chelation in prostate cancer." in: Scientific reports, Vol. 7, Issue 1, pp. 11632, (2017) (PubMed).

Huang, Zhang, Guo, Huang, Yang, Chen, Huang, Zhang, Long, Yang: "Honokiol protects against doxorubicin cardiotoxicity via improving mitochondrial function in mouse hearts." in: Scientific reports, Vol. 7, Issue 1, pp. 11989, (2017) (PubMed).

Wang, Tian, Chen, Tan, Xie, Chen, Fu, Chen, Xu, Wei: "Low dose doxycycline decreases systemic inflammation and improves glycemic control, lipid profiles, and islet morphology and function in db/db mice." in: Scientific reports, Vol. 7, Issue 1, pp. 14707, (2017) (PubMed).

Qu, Bing, Meng, Xi, Bai, Liu, Guo, Zhao, Bi: "Upregulation of miR-126-3p promotes human saphenous vein endothelial cell proliferation in vitro and prevents vein graft neointimal formation ex vivo and in vivo." in: Oncotarget, Vol. 8, Issue 63, pp. 106790-106806, (2017) (PubMed).

Wang, Zhang, Chai, Liu, Li, Wang, Zhu, Cui, Qu, Zhu: "1,25(OH)2D3 downregulates the Toll-like receptor 4-mediated inflammatory pathway and ameliorates liver injury in diabetic rats." in: Journal of endocrinological investigation, Vol. 38, Issue 10, pp. 1083-91, (2016) (PubMed).

Yan, Fan, Bai, Hou, Li, Tong: "Zinc Prevents Abdominal Aortic Aneurysm Formation by Induction of A20-Mediated Suppression of NF-κB Pathway." in: PLoS ONE, Vol. 11, Issue 2, pp. e0148536, (2016) (PubMed).

Target Details for CD68

Target: CD68 (CD68 Molecule (CD68))

Alternative Name: CD68 (CD68 Products)

Synonyms: Lamp4 antibody, Scard1 antibody, gp110 antibody, GP110 antibody, LAMP4 antibody, SCARD1 antibody, CD68 molecule antibody, CD68 antigen antibody, Cd68 molecule antibody, CD68 antibody, Cd68 antibody

Background: CD68, cluster of differentiation, is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. CD68 is a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34. The CD68 gene is mapped to 17p13.1. Immunohistochemistry can be used to identify the presence of CD68, which is found in the cytoplasmic granules of a range of different blood cells. It is particularly useful as a marker for the various cells of the macrophage lineage, including monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts. This allows it to be used to distinguish diseases of otherwise similar appearance, such as the monocyte/macrophage and lymphoid forms of leukaemia(the latter being CD68 negative). Its presence in macrophages also makes it useful in diagnosing conditions related to proliferation or abnormality of these cells, such as malignant histiocytosis, histiocytic lymphoma, and Gaucher's disease.

Synonyms: CD 68 antibody|CD68 antibody|CD68 antigen antibody|CD68 Molecule antibody|CD68_HUMAN antibody|DKFZp686M18236 antibody|GP110 antibody|LAMP4 antibody|Macrophage antigen CD68(microsialin) antibody|MACROPHAGE ANTIGEN CD68 antibody|macrosialin antibody|SCARD1 antibody|Scavenger receptor class D member 1 antibody

UniProt: P31996