N-Cadherin antibody (Middle Region)

Catalog No. ABIN3044370

Product Details anti-N-Cadherin Antibody

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Binding Specificity: AA 701-714, Middle Region

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This N-Cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Cadherin-2(CDH2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: CQCDSNGDCT DVDR

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Cadherin-2(CDH2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: cadherin 2, type 1, N-cadherin(neuronal)
Protein Name: Cadherin-2

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human N Cadherin(701-714aa CQCDSNGDCTDVDR), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-N-Cadherin antibody (ABIN3044370)

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Shan, Lv, Zhao, Liu, Sun, Xi, Xiao, Li: "Wnt/β-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells." in: International journal of clinical and experimental pathology, Vol. 8, Issue 10, pp. 12357-67, (2016) (PubMed).

Lin, Sun, Wang, Zheng, Zhang, Zheng: "Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition." in: Frontiers in pharmacology, Vol. 6, pp. 320, (2016) (PubMed).

Tong, Sun, Wang, Suo: "Sorcin Enhances Metastasis and Promotes Epithelial-to-Mesenchymal Transition of Colorectal Cancer." in: Cell biochemistry and biophysics, (2015) (PubMed).

Liu, Liu, Jiang, Peng, Ma, Lu: "SOX9 Overexpression Promotes Glioma Metastasis via Wnt/?-Catenin Signaling." in: Cell biochemistry and biophysics, (2015) (PubMed).

Sun, Hu, Zheng, Bagnoli, Guo, Rupaimoole, Rodriguez-Aguayo, Lopez-Berestein, Ji, Chen, Sood, Mezzanzanica, Liu, Sun, Zhang: "MiR-506 inhibits multiple targets in the epithelial-to-mesenchymal transition network and is associated with good prognosis in epithelial ovarian cancer." in: The Journal of pathology, Vol. 235, Issue 1, pp. 25-36, (2015) (PubMed).

Su, Li: "RAC1 overexpression promotes the proliferation, migration and epithelial-mesenchymal transition of lens epithelial cells." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 10760-7, (2015) (PubMed).

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Kudo-Sakamoto, Akazawa, Ito, Takano, Yano, Yabumoto, Naito, Oka, Lee, Sakata, Suzuki, Saido, Komuro: "Calpain-dependent cleavage of N-cadherin is involved in the progression of post-myocardial infarction remodeling." in: The Journal of biological chemistry, Vol. 289, Issue 28, pp. 19408-19, (2014) (PubMed).

Wang, Li, Zhou, Sun, Wang, Cheng, Ma, Liu, Liu, Wang, Liang, Wu: "Intervention effects of ganoderma lucidum spores on epileptiform discharge hippocampal neurons and expression of neurotrophin-4 and N-cadherin." in: PLoS ONE, Vol. 8, Issue 4, pp. e61687, (2013) (PubMed).

Qiao, Johnson, Gao et al.: "Epithelial-mesenchymal transition in oral squamous cell carcinoma triggered by transforming growth factor-beta1 is Snail family-dependent and correlates with matrix metalloproteinase-2 and -9 ..." in: International journal of oncology, Vol. 37, Issue 3, pp. 663-8, (2010) (PubMed).

Target Details for N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Alternative Name: CDH2 (CDH2 Products)

Synonyms: CD325 antibody, CDHN antibody, CDw325 antibody, NCAD antibody, N-cadherin antibody, cadherin-2 antibody, cdhn antibody, ncad antibody, cdw325 antibody, CDH2 antibody, Cadherin-2 antibody, Ncad antibody, glo antibody, lyr antibody, pac antibody, wu:fb47h04 antibody, cadherin 2 antibody, cadherin 2 S homeolog antibody, cadherin 2, type 1, N-cadherin (neuronal) antibody, CDH2 antibody, cdh2.S antibody, cdh2 antibody, Cdh2 antibody

Background: N-cadherin(NCAD) is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule(L-CAM). N-Cadherin, also known as Cadherin-2, encodes a 907-amino acid protein that includes a 159-amino acid signal sequence. Human and mouse nucleotide sequences are 96 % identical. Mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. Human N-cadherin gene contains 16 exons and its sequence is highly similar to both the mouse NCAD gene(including the large first and second introns) and other cadherin genes. N-cadherin is mapped to 18q11.2. Cadherin regulates dendritic spine morphogenesis.

Synonyms: CADH2_HUMAN antibody|Cadherin 2 antibody|Cadherin 2 N cadherin neuronal antibody|Cadherin 2 type 1 antibody|Cadherin 2 type 1 N cadherin neuronal antibody|cadherin 2 type 1 N-cadherin neuronal antibody|Cadherin 2, type 1, N-cadherin(neuronal) antibody|Cadherin-2 antibody|Cadherin2 antibody|Calcium dependent adhesion protein neuronal antibody|CD325 antibody|CD325 antigen antibody|CDH2 antibody|CDHN antibody|CDw325 antibody|CDw325 antigen antibody|N cadherin 1 antibody|N-cadherin antibody|NCAD antibody|Neural Cadherin antibody|OTTHUMP00000066304 antibody|OTTHUMP00000067378 antibody

UniProt: P19022

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane