MORG1 antibody (N-Term)

Catalog No. ABIN3044326

Product Details anti-MORG1 Antibody

Target: MORG1 (wdr83) (WD Repeat Domain 83 (wdr83))

Binding Specificity: AA 29-43, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MORG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for WD repeat domain-containing protein 83(WDR83) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: RAVRFNVDGN YCLTC

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for WD repeat domain-containing protein 83(WDR83) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: WD repeat domain 83
Protein Name: WD repeat domain-containing protein 83

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Morg1(29-43aa RAVRFNVDGNYCLTC), identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for MORG1

Target: MORG1 (wdr83) (WD Repeat Domain 83 (wdr83))

Alternative Name: WDR83 (wdr83 Products)

Synonyms: MORG1 antibody, 1500041N16Rik antibody, Morg1 antibody, RGD1306947 antibody, morg1 antibody, MAPK organizer 1 antibody, wu:fj29g04 antibody, zgc:92654 antibody, WD repeat domain 83 antibody, WD repeat domain containing 83 antibody, WD repeat domain 83 L homeolog antibody, WDR83 antibody, Wdr83 antibody, wdr83.L antibody, wdr83 antibody

Background: MORG1(mitogen-activated protein kinase organizer 1), a member of the WD-40 protein family that was isolated as a binding partner of the extracellular signal-regulated kinase(ERK) pathway scaffold protein MP1. MORG1 specifically associates with several components of the ERK pathway, including MP1, Raf-1, MEK, and ERK, and stabilizes their assembly into an oligomeric complex. MORG1 facilitates ERK activation when cells are stimulated with lysophosphatidic acid, phorbol 12-myristate 13-acetate, or serum, but not in response to epidermal growth factor. Suppression of MORG1 by short interfering RNA leads to a marked reduction in ERK activity when cells are stimulated with serum. MORG1 is a component of a modular scaffold system that participates in the regulation of agonist-specific ERK signaling.

Synonyms: MAPK organizer 1 antibody|MGC4238 antibody|Mitogen activated protein kinase organizer 1 antibody|Mitogen-activated protein kinase organizer 1 antibody|MORG 1 antibody|WD repeat domain-containing protein 83 antibody|wdr83 antibody|WDR83_HUMAN antibody