Integrin alpha 1 antibody (C-Term)

Catalog No. ABIN3044325

Product Details anti-Integrin alpha 1 Antibody

Target: Integrin alpha 1 (ITGA1)

Binding Specificity: AA 1121-1134, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Integrin alpha 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Integrin alpha-1(ITGA1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: SNQKRELAIQ ISKD

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Integrin alpha-1(ITGA1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: integrin, alpha 1
Protein Name: Integrin alpha-1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Integrin alpha 1(1121-1134aa SNQKRELAIQISKD), different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-Integrin alpha 1 antibody (ABIN3044325)

Zhang, Ren, Luo, Fan, Liu, Liang, Ma, Yu, Bai: "A small physiological electric field mediated responses of extravillous trophoblasts derived from HTR8/SVneo cells: involvement of activation of focal adhesion kinase signaling." in: PLoS ONE, Vol. 9, Issue 3, pp. e92252, (2014) (PubMed).

Target Details for Integrin alpha 1

Target: Integrin alpha 1 (ITGA1)

Alternative Name: ITGA1 (ITGA1 Products)

Synonyms: CD49A antibody, E130012M19Rik antibody, Vla1 antibody, CD49a antibody, VLA1 antibody, integrin alpha 1 antibody, integrin subunit alpha 1 antibody, Itga1 antibody, ITGA1 antibody

Background: Integrin alpha 1(ITGA1) chain associates with the beta 1(ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.

Synonyms: CD 49a antibody|CD49 antigen-like family member A antibody|CD49a antibody|CD49a antigen antibody|Integrin alpha-1 antibody|ITA1_HUMAN antibody|Itga 1 antibody|ITGA1 antibody|Laminin and collagen receptor antibody|Very late activation protein 1 antibody|VLA 1 antibody|VLA-1 antibody|VLA1 antibody

UniProt: P56199

Pathways: EGFR Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex