CCR5 antibody (N-Term)

Catalog No. ABIN3044318

Product Details anti-CCR5 Antibody

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Binding Specificity: AA 19-34, N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCR5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for C-C chemokine receptor type 5(CCR5) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: PCQKINVKQI AARLLP

Cross-Reactivity (Details): Predicted Cross Reactivity: rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for C-C chemokine receptor type 5(CCR5) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: chemokine(C-C motif) receptor 5(gene/pseudogene)
Protein Name: C-C chemokine receptor type 5(C-C CKR-5/CC-CKR-5/CCR-5/CCR5)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CCR5(19-34aa PCQKINVKQIAARLLP), different from the related rat sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Predicted Species: Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-CCR5 antibody (ABIN3044318)

Li, Xu, Zhu, Xu, Qin, Liu, Tu, Peng, Qiu, Yu, Zhu, Fan, Zheng, Li, Liang: "Effects of neferine on CCL5 and CCR5 expression in SCG of type 2 diabetic rats." in: Brain research bulletin, Vol. 90, pp. 79-87, (2012) (PubMed).

Target Details for CCR5

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Alternative Name: CCR5 (CCR5 Products)

Synonyms: CC-CKR-5 antibody, CCCKR5 antibody, CCR-5 antibody, CD195 antibody, CKR-5 antibody, CKR5 antibody, CMKBR5 antibody, IDDM22 antibody, Ckr5 antibody, Cmkbr5 antibody, CCR1-L antibody, GPCR antibody, CCR5 antibody, AM4-7 antibody, C-C motif chemokine receptor 5 (gene/pseudogene) antibody, chemokine (C-C motif) receptor 5 antibody, C-C motif chemokine receptor 5 antibody, CCR5 antibody, Ccr5 antibody

Background: Human CC chemokine receptor 5(CCR5), which is 17.5 kb from the CMKBR2 gene, encodes a 352-amino acid protein with a calculated molecular mass of 40,600 Da. It is organized into four exons and two introns. Human CCR5 is a co-receptor for macrophage-tropic strains of human immunodeficiency virus(HIV)-1 and is expressed by bone-marrow-derived cells. CCR5 is expressed on neurons, astrocytes, and microglia in the central nervous system, and on epithelium, endothelium, vascular smooth muscle and fibroblasts in other tissues. Functionally, CCR5 mediates the activation of cells by the chemokines macrophage inflammatory protein-1alpha, macrophage inflammatory protein-1beta, and RANTES, and serves as a fusion cofactor for macrophage-tropic strains of human immunodeficiency virus type 1.

Synonyms: AM4 7 antibody|C C chemokine receptor type 5 antibody|C C CKR 5 antibody|C-C chemokine receptor type 5 antibody|C-C CKR-5 antibody|C-C motif chemokine receptor 5 A159A antibody|CC Chemokine Receptor 5 antibody|CC Chemokine Receptor Type 5 antibody|CC CKR 5 antibody|CC-CKR-5 antibody|CCCKR 5 antibody|CCCKR5 antibody|CCR 5 antibody|CCR-5 antibody|CCR5 antibody|CCR5 chemokine(C C motif) receptor 5 antibody|CCR5_HUMAN antibody|CD 195 antibody|CD195 antibody|CD195 Antigen antibody|Chemokine C C motif receptor 5 antibody|Chemokine receptor CCR5 antibody|CHEMR13 antibody|CKR 5 antibody|CKR5 antibody|CMKBR 5 antibody|CMKBR5 antibody|FLJ78003 antibody|HIV 1 Fusion Coreceptor antibody|HIV-1 fusion coreceptor antibody|HIV1 fusion coreceptor antibody|IDDM22 antibody|MIP-1 alpha receptor antibody

UniProt: P51681

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Cell Size