Annexin V antibody (Middle Region)

Catalog No. ABIN3044315

Product Details anti-Annexin V Antibody

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Binding Specificity: AA 88-102, Middle Region

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Annexin V antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Annexin A5(ANXA5) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Sequence: SRLYDAYELK HALKG

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Annexin A5(ANXA5) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: annexin A5
Protein Name: Annexin A5

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Annexin V(88-102aa SRLYDAYELKHALKG), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-Annexin V antibody (ABIN3044315)

Manville, Abbott: "Ancient and modern anticonvulsants act synergistically in a KCNQ potassium channel binding pocket." in: Nature communications, Vol. 9, Issue 1, pp. 3845, (2018) (PubMed).

Sun, Kang, Yao, Chen, Sun, An, Jiang, Wang, Hu: "Dehydrocostus lactone suppressed the proliferation, migration, and invasion of colorectal carcinoma through the downregulation of eIF4E expression." in: Anti-cancer drugs, Vol. 26, Issue 6, pp. 641-8, (2015) (PubMed).

Ji, Li, Wei, Kong, Jin, Chen, Li, Deng: "Effect of celastrol on growth inhibition of prostate cancer cells through the regulation of hERG channel in vitro." in: BioMed research international, Vol. 2015, pp. 308475, (2015) (PubMed).

Xu, Bi, Li, Fang, Zhou, Li, Chi, Wan, Shen: "Morphological and proteomic analyses reveal that unsaturated guluronate oligosaccharide modulates multiple functional pathways in murine macrophage RAW264.7 cells." in: Marine drugs, Vol. 13, Issue 4, pp. 1798-818, (2015) (PubMed).

Hu, Dong, Shi, Deng, Li, Lu: "Evaluation of a novel tetra-functional branched poly(ethylene glycol) crosslinker for manufacture of crosslinked, decellularized, porcine aortic valve leaflets." in: Journal of biomedical materials research. Part B, Applied biomaterials, Vol. 102, Issue 2, pp. 322-36, (2014) (PubMed).

Xiong, Ma, Zhang, Shao, Zhang, Zhou: "Apoptosis induced by ?,?-dimethylacrylshikonin is associated with Bcl-2 and NF-?B in human breast carcinoma MCF-7 cells." in: Oncology letters, Vol. 6, Issue 6, pp. 1789-1793, (2013) (PubMed).

Jin, Liu, Wang, Xu, Yang, Lu, Sun, Hu: "Protective effects of [Gly14]-Humanin on beta-amyloid-induced PC12 cell death by preventing mitochondrial dysfunction." in: Neurochemistry international, Vol. 56, Issue 3, pp. 417-23, (2010) (PubMed).

Target Details for Annexin V

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Alternative Name: ANXA5 (ANXA5 Products)

Synonyms: anx antibody, anx5 antibody, ANX V antibody, anxa5 antibody, cb989 antibody, wu:fa98f06 antibody, wu:fj10f10 antibody, MGC89158 antibody, ANX5 antibody, ENX2 antibody, PP4 antibody, RPRGL3 antibody, Anx5 antibody, R74653 antibody, LC5 antibody, enx2 antibody, annexin A5 antibody, annexin A5b antibody, Annexin A5 antibody, annexin A5 L homeolog antibody, ANXA5 antibody, anxa5b antibody, anxa5 antibody, Anxa5 antibody, anxa5.L antibody

Background: Annexin V also know as endonnexin II(ENX2),or placental protein 4(PP4). Endonexin II is a member of the family of Ca(2+)-dependent phospholipid binding proteins, known as annexins. It bind to the phospholipids that are preferentially located on the cytosolic face of the plasma membrane. It has a relative molecular weight of about 35,000. The gene lies on mouse chromosome 3 in close linkage with the fibroblast growth factor 2(basic) gene and is syntenic with other genes known to have orthologous counterparts on human chromosome 4q. The PP4 cDNA encoded a protein of 320 amino acid residues. A single mRNA, approximately 1.6 kb long, was found to be expressed in human cell lines and placenta. PP4 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Synonyms: Anchorin CII antibody|Annexin 5 antibody|Annexin A5 antibody|Annexin V antibody|Annexin-5 antibody|Annexin5 antibody|AnnexinA5 antibody|AnnexinV antibody|ANX 5 antibody|ANX A5 antibody|ANX5 antibody|ANXA5 antibody|ANXA5_HUMAN antibody|Calphobindin I antibody|CBP I antibody|CBP-I antibody|Endonexin II antibody|ENX 2 antibody|ENX2 antibody|Lipocortin V antibody|PAP I antibody|PAP-I antibody|Placental anticoagulant protein 4 antibody|Placental anticoagulant protein I antibody|PLACENTAL PROTEIN 4 antibody|PP 4 antibody|PP4 antibody|Thromboplastin inhibitor antibody|VAC alpha antibody|VAC-alpha antibody|Vascular anticoagulant alpha antibody|Vascular anticoagulant-alpha antibody

UniProt: P08758

Pathways: Apoptosis