ALPL antibody (N-Term)

Catalog No. ABIN3044314

Product Details anti-ALPL Antibody

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Binding Specificity: AA 21-35, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALPL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Alkaline phosphatase, tissue-nonspecific isozyme(ALPL) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: EKEKDPKYWR DQAQE

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Alkaline phosphatase, tissue-nonspecific isozyme(ALPL) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: alkaline phosphatase, liver/bone/kidney
Protein Name: Alkaline phosphatase, tissue-nonspecific isozyme(AP-TNAP/TNSALP)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Alkaline Phosphatase(21-35aa,EKEKDPKYWRDQAQE), different from the related rat and mouse sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-ALPL antibody (ABIN3044314)

Sun, Jiang, Chen, Xue, Mao, Ruan, Song, Mustea: "Decreasing the ratio of matriptase/HAI‑1 by downregulation of matriptase as a potential adjuvant therapy in ovarian cancer." in: Molecular medicine reports, Vol. 14, Issue 2, pp. 1465-74, (2017) (PubMed).

Zhang, Wei, Ding, Zhang, Wang, Zhu, He, Chai, Liu: "Increased microRNA-93-5p inhibits osteogenic differentiation by targeting bone morphogenetic protein-2." in: PLoS ONE, Vol. 12, Issue 8, pp. e0182678, (2017) (PubMed).

Hou, Huang, Luo, Wang, Liu, Deng, Zhang, Liu, Chen: "MiR-351 negatively regulates osteoblast differentiation of MSCs induced by (+)-cholesten-3-one through targeting VDR." in: American journal of translational research, Vol. 9, Issue 11, pp. 4963-4973, (2017) (PubMed).

Liu, Deng, Zhang, Zhang: "Wnt3a expression during the differentiation of adipose-derived stem cells into cholinergic neurons." in: Neural regeneration research, Vol. 7, Issue 19, pp. 1463-8, (2015) (PubMed).

Xu, Dong, Huang, Li, Qin, Ren, Guo, Chen, Huang: "Decreased osteoclastogenesis, osteoblastogenesis and low bone mass in a mouse model of type 2 diabetes." in: Molecular medicine reports, Vol. 10, Issue 4, pp. 1935-41, (2014) (PubMed).

Liu, Lu, Wu, Yan: "A multi-biomarker assessment of single and combined effects of norfloxacin and sulfamethoxazole on male goldfish (Carassius auratus)." in: Ecotoxicology and environmental safety, Vol. 102, pp. 12-7, (2014) (PubMed).

Guo, Shi, Liu, Lin, Bi, Shi, Yan: "Cartilage oligomeric matrix protein gene multilayers inhibit osteogenic differentiation and promote chondrogenic differentiation of mesenchymal stem cells." in: International journal of molecular sciences, Vol. 15, Issue 11, pp. 20117-33, (2014) (PubMed).

Feng, Zhu, Zhang, Jia, Cheng, Ding, Zhu et al.: "Amelioration of compound 4,4'-diphenylmethane-bis(methyl)carbamate on high mobility group box1-mediated inflammation and oxidant stress responses in human umbilical vein endothelial cells via ..." in: International immunopharmacology, Vol. 15, Issue 2, pp. 206-16, (2013) (PubMed).

Shen, Zhan, Deng, Ren, Ye, Xiao, Guo: "MicroRNA-519a demonstrates significant tumour suppressive activity in laryngeal squamous cells by targeting anti-carcinoma HuR gene." in: The Journal of laryngology and otology, Vol. 127, Issue 12, pp. 1194-202, (2013) (PubMed).

Zhang, Wu, Hu, Wang, Li: "Rho kinase inhibitor Y-27632 and Accutase dramatically increase mouse embryonic stem cell derivation." in: In vitro cellular & developmental biology. Animal, Vol. 48, Issue 1, pp. 30-6, (2012) (PubMed).

Zhang, Yang, Jiang, Fang, Xiong, Cheng, Xia: "The lupus-derived anti-double-stranded DNA IgG contributes to myofibroblast-like phenotype in mesangial cells." in: Journal of clinical immunology, Vol. 32, Issue 6, pp. 1270-8, (2012) (PubMed).

Li, Xia, Tang, Wang, Bei, Zeng: "In vitro and in vivo biocompatibility investigation of diamond-like carbon coated nickel-titanium shape memory alloy." in: Artificial cells, blood substitutes, and immobilization biotechnology, Vol. 39, Issue 3, pp. 137-42, (2011) (PubMed).

Su, Wang, Qi, Zhao, Li, Cui: "Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts." in: Molecular vision, Vol. 17, pp. 247-56, (2011) (PubMed).

Zhao, Gao, Mei, Li, Wang: "Differentiation of human mesenchymal stem cells: the potential mechanism for estrogen-induced preferential osteoblast versus adipocyte differentiation." in: The American journal of the medical sciences, Vol. 341, Issue 6, pp. 460-8, (2011) (PubMed).

Wang, Huang, Feng, Chen, Tang, Chen: "Expression, purification, and characterization of functional recombinant human daintain/AIF-1 in Escherichia coli." in: Zeitschrift fu?r Naturforschung. C, Journal of biosciences, Vol. 65, Issue 11-12, pp. 726-32, (2011) (PubMed).

Lai, Yan, Wu, Leng, Ju: "Ultrasensitive multiplexed immunoassay with electrochemical stripping analysis of silver nanoparticles catalytically deposited by gold nanoparticles and enzymatic reaction." in: Analytical chemistry, Vol. 83, Issue 7, pp. 2726-32, (2011) (PubMed).

Shen, Ren, Ye, Guo, Kang, Ding: "Significance and relationship between DJ-1 gene and surviving gene expression in laryngeal carcinoma." in: European journal of histochemistry : EJH, Vol. 55, Issue 1, pp. e9, (2011) (PubMed).

Guan, Wang, Hou, Chen, Li, Yue, Ma: "Derivation and characteristics of pluripotent embryonic germ cells in duck." in: Poultry science, Vol. 89, Issue 2, pp. 312-7, (2010) (PubMed).

Song, Lu, Wang, Zhang, Xu, Qin: "Study on environmental estrogen pollution in Yangtze River (Nanjing section) by an in vivo bioassay." in: Bulletin of environmental contamination and toxicology, Vol. 84, Issue 4, pp. 406-12, (2010) (PubMed).

Lu, Song, Wang, Yan: "Assessment of in vivo estrogenic response and the identification of environmental estrogens in the Yangtze River (Nanjing section)." in: Chemosphere, Vol. 80, Issue 9, pp. 982-90, (2010) (PubMed).

Target Details for ALPL

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Alternative Name: ALPL (ALPL Products)

Synonyms: ALP antibody, iap antibody, alpl antibody, AP-TNAP antibody, APTNAP antibody, HOPS antibody, TNAP antibody, TNSALP antibody, Akp-2 antibody, Akp2 antibody, PHOA antibody, TNS-AP antibody, alkaline phosphatase, liver/bone/kidney L homeolog antibody, alkaline phosphatase, liver/bone/kidney antibody, alkaline phosphatase, intestinal, gene 2 antibody, alkaline phosphatase, liver/bone/kidney pseudogene antibody, alpl.L antibody, ALPL antibody, alpi.2 antibody, LOC100031702 antibody, Alpl antibody

Background: Alkaline phosphatase(ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH . Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific(found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts ,the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine(PEA) and pyridoxal-5-prime-phosphate(PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

Synonyms: Akp2 antibody|Alkaline phosphatase intestinal antibody|Alkaline phosphatase intestinal precursor antibody|Alkaline phosphatase liver/bone/kidney antibody|Alkaline phosphatase placental antibody|Alkaline phosphatase placental like 2 antibody|Alkaline phosphatase placental like antibody|Alkaline phosphatase placental type antibody|Alkaline phosphomonoesterase antibody|ALP 1 antibody|ALP antibody|ALP I antibody|ALP L antibody|ALP P antibody|ALPG antibody|ALPI antibody|ALPL antibody|ALPP antibody|ALPPL antibody|ALPPL2 antibody|AP TNAP antibody|FLJ40094 antibody|GCAP antibody|Germ cell alkaline phosphatase antibody|Glycerophosphatase antibody|HOPS antibody|IAP antibody|Intestinal alkaline phosphatase antibody|Kasahara isozyme antibody|Nagao isozyme antibody|OTTHUMP00000164357 antibody|Placental alkaline phosphatase antibody|Placental like alkaline phosphatase antibody|PLAP 1 antibody|PLAP antibody|PLAP like antibody|Regan isozyme antibody|Testicular and thymus alkaline phosphatase antibody|Tissue non specific alkaline phosphatase antibody|Tissue nonspecific ALP antibody|TNAP antibody|TNSALP antibody

UniProt: P05186