Hsc70 antibody (N-Term)

Catalog No. ABIN3044300

The Rabbit Polyclonal anti-Hsc70 antibody has been validated for WB, IHC, ICC, IF, FACS and IHC (fro). It is suitable to detect Hsc70 in samples from Human, Rat and Mouse. There are 5+ publications available.

Quick Overview for Hsc70 antibody (N-Term) (ABIN3044300)

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hsc70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Hsc70 Antibody

Binding Specificity: AA 13-31, N-Term

Purpose: Anti-Hsp70/HSPA1A/HSPA1B Antibody Picoband®

Sequence: TTYSCVGVFQ HGKVEIIAN

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Hsp70/HSPA1A/HSPA1B Antibody (ABIN3044300). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Hsp70, identical to the related rat and mouse sequence.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Mouse, Rat
Immunocytochemistry , 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Tavaria M, Gabriele T, Kola I, Anderson RL (April 1996). "A hitchhiker's guide to the human Hsp70 family". Cell Stress Chaperones 1 (1): 23-8. 2. Morano KA (October 2007). "New tricks for an old dog: the evolving world of Hsp70". Ann. N. Y. Acad. Sci. 1113: 1-14. 3. Luders, J., Demand, J., Hohfeld, J. (2000), Journal of Biological Chemistry 275 (7): 4613-461, http://www.jbc.org/cgi/content/full/275/7/4613, retrieved on 2009-04-07

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Hsc70 antibody (ABIN3044300)

Zhang, Tang, Li, Li, Zhang, Yan, Cui: "Effects of intracerebral hemorrhage and subsequent minimally invasive hematoma aspiration on expression of apoptosisrelated genes in rats." in: International journal of clinical and experimental pathology, Vol. 8, Issue 5, pp. 5371-8, (2016) (PubMed).

Pu, Shi, Wang, Wang, Guo, Liu, Sun, Gao, Zhou: "Effects of minocycline on the expression of NGF and HSP70 and its neuroprotection role following intracerebral hemorrhage in rats." in: Journal of biomedical research, Vol. 25, Issue 4, pp. 292-8, (2013) (PubMed).

Li, Roubeix, Wang, Shi, Liu, Baudouin, Chen: "Therapeutic efficacy of trehalose eye drops for treatment of murine dry eye induced by an intelligently controlled environmental system." in: Molecular vision, Vol. 18, pp. 317-29, (2012) (PubMed).

Yi, Peng, Chang, Peng, Yan, Lin: "Effect of pre-moxibustion on apoptosis and proliferation of gastric mucosa cells." in: World journal of gastroenterology, Vol. 13, Issue 15, pp. 2174-8, (2007) (PubMed).

Wu, Cao, Gao, Chen, Wang, Zumbika, Qian: "Down-modulation of heat shock protein 70 and up-modulation of Caspase-3 during schisandrin B-induced apoptosis in human hepatoma SMMC-7721 cells." in: World journal of gastroenterology, Vol. 10, Issue 20, pp. 2944-8, (2004) (PubMed).

Target Details for Hsc70

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Alternative Name: HSPA8

Background:

Synonyms: Heat shock 70 kDa protein 1A; Heat shock 70 kDa protein 1B

Tissue Specificity: Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level). .

Background: The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. All of the Hsp70 proteins have three major functional domains: An N-terminal ATPase domain binds ATP (Adenosine triphosphate) and hydrolyzes it to ADP (Adenosine diphosphate); A substrate binding domain contains a groove with an affinity for neutral, hydrophobic amino acid residues; A C-terminal domain rich in alpha helical structure acts as a 'lid' for the substrate binding domain. By binding tightly to partially-synthesized peptide sequences (incomplete proteins), Hsp70 prevents them from aggregating and being rendered nonfunctional. And it also can act to protect cells from thermal or oxidative stress. Finally, Hsp70 seems to be able to participate in disposal of damaged or defective proteins. Interaction with CHIP (Carboxyl-terminus of Hsp70 Interacting Protein)–an E3 ubiquitin ligase–allows Hsp70 to pass proteins to the cell's ubiquitination and proteolysis pathways.

Sequence Similarities: Belongs to the beta-catenin family.

Molecular Weight: 70 kDa

UniProt: P08P09