HSD17B4 antibody (C-Term)

Catalog No. ABIN3044284

Product Details anti-HSD17B4 Antibody

Target: HSD17B4 (Hydroxysteroid (17-Beta) Dehydrogenase 4 (HSD17B4))

Binding Specificity: AA 744-761, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD17B4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Peroxisomal multifunctional enzyme type 2(HSD17B4) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: NIMLSQKLQM ILKDYAKL

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Peroxisomal multifunctional enzyme type 2(HSD17B4) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: hydroxysteroid(17-beta) dehydrogenase 4
Protein Name: Peroxisomal multifunctional enzyme type 2

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Hydroxysteroid(17-beta) Dehydrogenase 4(744-761aa NIMLSQKLQMILKDYAKL), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HSD17B4

Target: HSD17B4 (Hydroxysteroid (17-Beta) Dehydrogenase 4 (HSD17B4))

Alternative Name: HSD17B4 (HSD17B4 Products)

Synonyms: zgc:55545 antibody, zgc:77300 antibody, CG3415 antibody, DmMFE-2 antibody, Dmel\\CG3415 antibody, MFE-2 antibody, DBP antibody, MPF-2 antibody, PRLTS1 antibody, SDR8C1 antibody, 17-beta-HSD antibody, 17[b]-HSD antibody, 4 antibody, MFP2 antibody, Mfp-2 antibody, perMFE-2 antibody, hydroxysteroid (17-beta) dehydrogenase 4 antibody, hydroxysteroid 17-beta dehydrogenase 4 antibody, peroxisomal Multifunctional enzyme type 2 antibody, hsd17b4 antibody, HSD17B4 antibody, Mfe2 antibody, Hsd17b4 antibody

Background: Peroxisomal multifunctional enzyme type 2 is a protein that in humans is encoded by the HSD17B4 gene. The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Synonyms: 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase antibody|17 beta HSD 4 antibody|17 beta HSD IV antibody|17 beta hydroxysteroid dehydrogenase 4 antibody|17-beta-HSD 4 antibody|17-beta-hydroxysteroid dehydrogenase 4 antibody|17beta estradiol dehydrogenase type IV antibody|3 alpha 7 alpha12 alpha trihydroxy 5 beta cholest 24 enoyl CoA hydratase antibody|3-alpha antibody|7-alpha antibody|Beta hydroxyacyl dehydrogenase antibody|Beta keto reductase antibody|D 3 hydroxyacyl CoA dehydratase antibody|D bifunctional protein antibody|D bifunctional protein peroxisomal antibody|D-bifunctional protein antibody|DBP antibody|DHB4_HUMAN antibody|EDH17B4 antibody|Enoyl-CoA hydratase 2 antibody|Hsd17b4 antibody|MFE 2 antibody|MFE-2 antibody|MPF-2 antibody|Multifunctional protein 2 antibody|Peroxisomal multifunctional enzyme type 2 antibody|Peroxisomal multifunctional protein 2 antibody|SDR8C1 antibody|Short chain dehydrogenase/reductase family 8C member 1 antibody

Pathways: Monocarboxylic Acid Catabolic Process