FGF21 antibody (N-Term)

Catalog No. ABIN3044256

Product Details anti-FGF21 Antibody

Target: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Binding Specificity: AA 43-58, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGF21 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Fibroblast growth factor 21(FGF21) detection. Tested with WB in Human,Mouse,Rat.

Sequence: QVRQRYLYTD DAQQTE

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Fibroblast growth factor 21(FGF21) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: fibroblast growth factor 21
Protein Name: Fibroblast growth factor 21(FGF-21)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human FGF21(43-58aa QVRQRYLYTDDAQQTE), different from the related rat and mouse sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Predicted Species: Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for FGF21

Target: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Alternative Name: FGF21 (FGF21 Products)

Synonyms: FGF21 antibody, fibroblast growth factor 21 antibody, FGF21 antibody, fgf21 antibody, Fgf21 antibody

Background: FGF21(Fibroblast growth factor 21) is a protein that in humans is encoded by the FGF21 gene, which is also a member of the fibroblast growth factor(FGF) family. The FGF21 gene is mapped on 19q13.33. Using RT-PCR, Fgf21 was expressed in several types of adipose tissue in mice, including subcutaneous and epididymal fat pads and brown adipose tissue. The level of Fgf21 expression in adipose tissue was comparable to that in liver. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents. Changes in Fgf21 expression due to suckling or nutritional manipulations were associated with changes in circulating free fatty acid and ketone body levels. In differentiated mouse brown adipocytes in culture, Fgf21 treatment increased the expression of thermogenic genes, caused higher total and uncoupled respiration, and enhanced glucose oxidation.

Synonyms: FGF 21 antibody|FGF-21 antibody|Fgf21 antibody|FGF21_HUMAN antibody|FGFL antibody|Fibroblast growth factor 21 antibody|PRO10196 antibody|UNQ3115 antibody

Pathways: RTK Signaling