PRNP antibody (Middle Region)

Catalog No. ABIN3044180

Product Details anti-PRNP Antibody

Target: PRNP (Prion Protein (PRNP))

Binding Specificity: AA 143-159, Middle Region

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRNP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Major prion protein(PRNP) detection. Tested with WB, IHC-P in Mouse,Rat.

Sequence: DWEDRYYREN MYRYPNQ

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Major prion protein(PRNP) detection. Tested with WB, IHC-P in Mouse,Rat.
Gene Name: prion protein
Protein Name: Major prion protein(PrP)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse PRNP(143-159aa DWEDRYYRENMYRYPNQ), identical to the related rat sequence.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PRNP

Target: PRNP (Prion Protein (PRNP))

Alternative Name: PRNP (PRNP Products)

Synonyms: ASCR antibody, AltPrP antibody, CD230 antibody, CJD antibody, GSS antibody, KURU antibody, PRIP antibody, PrP antibody, PrP27-30 antibody, PrP33-35C antibody, PrPc antibody, p27-30 antibody, Prn antibody, Prn-p antibody, prp antibody, cjd antibody, prion antibody, prp(c) antibody, MGC84114 antibody, PRP antibody, PRPC antibody, Prp antibody, SIP antibody, PRNP antibody, AA960666 antibody, AI325101 antibody, PrP antibody, PrPC antibody, PrPSc antibody, Prn-i antibody, Sinc antibody, prP27-30 antibody, prP33-35C antibody, PrP-1 antibody, PrP1 antibody, prnprs1 antibody, stPrP-1 antibody, zgc:123004 antibody, prion protein antibody, prion protein S homeolog antibody, prion protein b antibody, PRNP antibody, Prnp antibody, prnp.S antibody, prnpb antibody

Background: PRNP(prion protein), also known as CD230 and PRP, is a protein that in humans is encoded by the PRNP gene. The major prion protein is expressed in the brain and several other tissues. Expression is most predominant in the nervous system but occurs in many other tissues throughout the body. Puckett et al.(1991)identified a RFLP with a high degree of heterozygosity in the 5-prime region of the PRNP gene, which might serve as a useful marker for the pter-p12 region of chromosome 20. PRNP is associated with a variety of cognitive deficiencies and neurodegenerative diseases such as Creutzfeldt-Jakob disease, bovine spongiform encephalopathy, and kuru. PRNP is highly conserved through mammals, lending credence to application of conclusions from test animals such as mice. Comparison between primates is especially similar, ranging from 92.9-99.6 % similarity in amino acid sequences.

Synonyms: Alternative prion protein, major prion protein antibody|AltPrP antibody|ASCR antibody|atal familial insomnia antibody|CD230 antibody|CD230 antigen antibody|CJD antibody|Creutzfeld Jakob disease antibody|Creutzfeld Jakob Disease Gerstmann Strausler Scheinker Syndrome Fatal Familial Insomnia antibody|Gerstmann-Strausler-Scheinker syndrome antibody|GSS antibody|KURU antibody|Major prion protein antibody|MGC26679 antibody|p27 30 antibody|PRIO_HUMAN antibody|Prion protein antibody|Prion related protein antibody|PRIP antibody|Prni antibody|Prnp antibody|PrP 27-30 antibody|PrP antibody|PrP27 30 antibody|PrP27-30 antibody|PrP33 35C antibody|PrP33-35C antibody|PrPC antibody|PrPSc antibody|Sinc antibody

UniProt: P04925

Pathways: Transition Metal Ion Homeostasis, Activated T Cell Proliferation