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IBSP antibody (C-Term)

IBSP Reactivity: Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN3044104
  • Target See all IBSP Antibodies
    IBSP (Integrin-Binding Sialoprotein (IBSP))
    Binding Specificity
    • 15
    • 13
    • 9
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 305-324, C-Term
    Reactivity
    • 57
    • 8
    • 8
    • 2
    • 1
    Mouse, Rat
    Host
    • 50
    • 4
    • 2
    • 1
    • 1
    Rabbit
    Clonality
    • 52
    • 6
    Polyclonal
    Conjugate
    • 27
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This IBSP antibody is un-conjugated
    Application
    • 49
    • 22
    • 13
    • 13
    • 13
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purpose
    Rabbit IgG polyclonal antibody for Bone sialoprotein 2(IBSP) detection. Tested with WB, IHC-P in Mouse,Rat.
    Sequence
    SYYKGHGYEG YEGQNYYYHQ
    Cross-Reactivity (Details)
    No cross reactivity with other proteins.
    Characteristics
    Rabbit IgG polyclonal antibody for Bone sialoprotein 2(IBSP) detection. Tested with WB, IHC-P in Mouse,Rat.
    Gene Name: integrin-binding sialoprotein
    Protein Name: Bone sialoprotein 2
    Purification
    Immunogen affinity purified.
    Immunogen
    A synthetic peptide corresponding to a sequence at the C-terminus of mouse Bone Sialoprotein(305-324aa SYYKGHGYEGYEGQNYYYHQ), different from the related rat sequence by one amino acid.
    Isotype
    IgG
    Top Product
    Discover our top product IBSP Primary Antibody
  • Application Notes
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Mouse, The detection limit for IBSP is approximately 2.5 ng/lane under reducing conditions.
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
    Preservative
    Thimerosal (Merthiolate), Sodium azide
    Precaution of Use
    This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
    Expiry Date
    12 months
  • Zhou, Deng, Liu, Li: "Effect of kidney-reinforcing and marrow-beneficial Chinese medicine on bone metabolism-related factors following spinal cord injury in rats." in: Experimental and therapeutic medicine, Vol. 12, Issue 1, pp. 485-491, (2016) (PubMed).

    Cheng, Wang, Zhang, Shi: "The osteogenic potential of mesoporous bioglasses/silk and non-mesoporous bioglasses/silk scaffolds in ovariectomized rats: in vitro and in vivo evaluation." in: PLoS ONE, Vol. 8, Issue 11, pp. e81014, (2014) (PubMed).

  • Target
    IBSP (Integrin-Binding Sialoprotein (IBSP))
    Alternative Name
    IBSP (IBSP Products)
    Synonyms
    BNSP antibody, BSP antibody, BSP-II antibody, SP-II antibody, Bsp antibody, BSP II antibody, BSPII antibody, Bsp2 antibody, IBSP antibody, integrin binding sialoprotein antibody, integrin-binding sialoprotein antibody, integrin-binding sialoprotein S homeolog antibody, IBSP antibody, Ibsp antibody, ibsp.S antibody, ibsp antibody
    Background
    IBSP(integrin-binding sialoprotein) is also known as BSP. The protein encoded by this gene is a major structural protein of the bone matrix. Bone sialoprotein is an acidic glycoprotein of approximately 70 kD that undergoes extensive posttranslational modifications. It constitutes approximately 12 % of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. The BSP gene is mapped to 4q22.1.

    Synonyms: BNSP antibody|Bone sialoprotein 2 antibody|Bone sialoprotein II antibody|BSP antibody|BSP II antibody|BSPII antibody|Cell binding sialoprotein antibody|Cell-binding sialoprotein antibody|IBSP antibody|Integrin binding sialoprotein antibody|Integrin-binding sialoprotein antibody|SIAL_HUMAN antibody|SPII antibody
    UniProt
    Q61711
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