FER antibody (Middle Region)

Catalog No. ABIN3044101

Product Details anti-FER Antibody

Target: FER (FER Tyrosine Kinase (FER))

Binding Specificity: AA 521-536, Middle Region

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FER antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Tyrosine-protein kinase Fer(FER) detection. Tested with WB, IHC-P in Human,Rat.

Sequence: FSNIPQLIDH HYTTKQ

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Tyrosine-protein kinase Fer(FER) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: fer(fps/fes related) tyrosine kinase
Protein Name: Tyrosine-protein kinase Fer

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human FER(521-536aa FSNIPQLIDHHYTTKQ), different from the related rat and mouse sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for FER is approximately 5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for FER

Target: FER (FER Tyrosine Kinase (FER))

Alternative Name: FER (FER Products)

Synonyms: FerT antibody, PPP1R74 antibody, TYK3 antibody, Fer antibody, Flk antibody, Flk_retired antibody, AV082135 antibody, C330004K01Rik antibody, Fert antibody, FER tyrosine kinase antibody, fer (fms/fps related) protein kinase antibody, FER antibody, Fer antibody

Background: FER(FPS/FES-Related tyrosine kinase) also known as TYK3, is an enzyme that in humans is encoded by the FER gene. Fer protein is a member of the FPS/FES family of nontransmembrane receptor tyrosine kinases. By in situ hybridization, Morris et al.(1990) concluded that the FER gene is located at 5q21-q22. Treatment of cells with JMP resulted in the release of FER from the cadherin complex and its accumulation in the integrin complex. The accumulation of FER in the integrin complex and the inhibitory effects of JMP could be reversed with a peptide that mimics the first coiled-coil domain of FER. The results suggested that FER mediates crosstalk between CDH2 and ITGB1. In Fer mutant mice, leukocyte emigration was exaggerated in response to LPS without altering vascular permeability, suggesting that FER has a role in regulating innate immunity.

Synonyms: c FER antibody|Fer(fps/fes related) tyrosine kinase(phosphoprotein NCP94) antibody|Fer(fps/fes related) tyrosine kinase antibody|FER antibody|FER_HUMAN antibody|p94 FER antibody|p94-FER antibody|Phosphoprotein NCP94 antibody|Proto oncogene tyrosine protein kinase FER antibody|Proto-oncogene c-Fer antibody|TYK3 antibody|Tyrosine kinase 3 antibody|Tyrosine-protein kinase Fer antibody

UniProt: P16591