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MBD4 antibody (C-Term)

MBD4 Reactivity: Human, Rat WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN3044062
  • Target See all MBD4 Antibodies
    MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))
    Binding Specificity
    • 9
    • 8
    • 8
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 566-580, C-Term
    Reactivity
    • 32
    • 5
    • 2
    • 1
    Human, Rat
    Host
    • 31
    • 1
    • 1
    Rabbit
    Clonality
    • 32
    • 1
    Polyclonal
    Conjugate
    • 16
    • 4
    • 4
    • 3
    • 3
    • 3
    This MBD4 antibody is un-conjugated
    Application
    • 26
    • 24
    • 10
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Purpose
    Rabbit IgG polyclonal antibody for Methyl-CpG-binding domain protein 4(MBD4) detection. Tested with WB in Human,Mouse,Rat.
    Sequence
    YHDWLWENHE KLSLS
    Cross-Reactivity (Details)
    Predicted Cross Reactivity: mouse
    No cross reactivity with other proteins.
    Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
    Characteristics
    Rabbit IgG polyclonal antibody for Methyl-CpG-binding domain protein 4(MBD4) detection. Tested with WB in Human,Mouse,Rat.
    Gene Name: methyl-CpG binding domain protein 4
    Protein Name: Methyl-CpG-binding domain protein 4
    Purification
    Immunogen affinity purified.
    Immunogen
    A synthetic peptide corresponding to a sequence at the C-terminus of human MBD4(566-580aa YHDWLWENHEKLSLS), identical to the related rat and mouse sequences.
    Isotype
    IgG
    Top Product
    Discover our top product MBD4 Primary Antibody
  • Application Notes
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for MBD4 is approximately 1 ng/lane under reducing conditions.
    Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
    Other applications have not been tested. Optimal dilutions should be determined by end users.
    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
    Preservative
    Thimerosal (Merthiolate), Sodium azide
    Precaution of Use
    This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
    Expiry Date
    12 months
  • Target
    MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))
    Alternative Name
    MBD4 (MBD4 Products)
    Synonyms
    MED1 antibody, Med1 antibody, methyl-CpG binding domain 4, DNA glycosylase antibody, methyl-CpG binding domain protein 4 antibody, methyl-CpG binding domain 4 DNA glycosylase antibody, MBD4 antibody, Mbd4 antibody
    Background
    MBD4(Methyl-CpG-Binding Domain Protein 4), also known as MED1, is a protein that in humans is encoded by the MBD4 gene. MBD4 specifically binds methylated DNA, colocalizes with methylated sequences, and is likely to mediate the effects of DNA methylation in mammalian cells(Hendrich and Bird, 1998). Riccio et al.(1999) mapped the MBD4 gene to chromosome 3q21-q22 by FISH. Hendrich and Bird(1998) found that both MBD2 and MBD4 specifically bound methylated DNA in vitro and colocalized with methylated sequences in vivo. They concluded that MBD2 and MBD4 are likely to be mediators of the effects of DNA methylation in mammalian cells. Hendrich et al.(1999) showed that MBD4 contains a methyl-CpG-binding domain that can efficiently remove thymine or uracil from mismatched CpG sites in vitro. Furthermore, the methyl-CpG-binding domain of MBD4 binds preferentially to 5-methylcytosine CpG-TpG mismatches--the primary product of deamination at methyl-CpG.

    Synonyms: 3 N(4) ethenocytosine glycosylase antibody|G/5 fluorouracil mismatch glycosylase with biphasic kinetics antibody|G/T mismatch glycosylase antibody|G/U mismatch glycosylase antibody|MBD 4 antibody|MBD4 antibody|MBD4_HUMAN antibody|MED 1 antibody|MED1 antibody|Methyl CpG binding domain protein 4 antibody|Methyl CpG binding endonuclease 1 antibody|Methyl CpG binding protein MBD4 antibody|Methyl-CpG-binding domain protein 4 antibody|Methyl-CpG-binding endonuclease 1 antibody|Methyl-CpG-binding protein MBD4 antibody|Mismatch specific DNA N glycosylase antibody|Mismatch-specific DNA N-glycosylase antibody
    UniProt
    O95243
    Pathways
    DNA Damage Repair
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