EIF2S1 antibody (Middle Region)
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- Target See all EIF2S1 Antibodies
- EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))
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Binding Specificity
- AA 123-137, Middle Region
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This EIF2S1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 2 subunit 1(EIF2S1) detection. Tested with WB, IHC-P in Human.
- Sequence
- KDEQLESLFQ RTAWV
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
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Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 2 subunit 1(EIF2S1) detection. Tested with WB, IHC-P in Human.
Gene Name: eukaryotic translation initiation factor 2, subunit 1 alpha, 35 kDa
Protein Name: Eukaryotic translation initiation factor 2 subunit 1 - Purification
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence in the middle region of human EIF2S1(123-137aa KDEQLESLFQRTAWV).
- Isotype
- IgG
- Top Product
- Discover our top product EIF2S1 Primary Antibody
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for EIF2S1 is approximately 0.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- Preservative
- Thimerosal (Merthiolate), Sodium azide
- Precaution of Use
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - Expiry Date
- 12 months
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- Target
- EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))
- Alternative Name
- EIF2S1 (EIF2S1 Products)
- Synonyms
- EIF-2 antibody, EIF-2A antibody, EIF-2alpha antibody, EIF2 antibody, EIF2A antibody, Eif2s1 antibody, eif2s1l antibody, fb36a08 antibody, wu:fb36a08 antibody, zgc:56510 antibody, 0910001O23Rik antibody, 2410026C18Rik antibody, 35kDa antibody, Eif2a antibody, eIF2alpha antibody, eif2a antibody, CG9946 antibody, Dmel\\CG9946 antibody, Eif2alpha antibody, IF2A_DROME antibody, M(1)14C antibody, M(1)19-153 antibody, M(1)8e3-10 antibody, deIF2alpha antibody, eIF-2 antibody, eIF-2 a antibody, eIF-2 alpha antibody, eIF2 alpha antibody, eIF2-alpha antibody, eIF2a antibody, eiF-2alpha antibody, elF2alpha antibody, l(1)14Cf antibody, l(1)19-153 antibody, eukaryotic translation initiation factor 2 subunit alpha antibody, eukaryotic translation initiation factor 2, subunit 1 alpha a antibody, eukaryotic translation initiation factor 2, subunit 1 alpha antibody, eukaryotic translation initiation factor 2 alpha subunit antibody, Eukaryotic translation initiation factor 2 subunit 1 antibody, eukaryotic translation initiation factor 2 subunit alpha L homeolog antibody, eukaryotic translation Initiation Factor 2alpha antibody, EIF2S1 antibody, eif2s1a antibody, Eif2s1 antibody, eif2s1 antibody, LOC693063 antibody, CNI00230 antibody, THAPSDRAFT_105 antibody, if2a antibody, eif2s1.L antibody, eIF-2alpha antibody
- Background
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EIF2S1(Eukaryotic Translation Initiation Factor 2, Subunit 1), also called EIF2-alpha, is a protein that in humans is encoded by the EIF2S1 gene. Hartz(2010) mapped the EIF2S1 gene to chromosome 14q23.3 based on an alignment of the EIF2S1 sequence with the genomic sequence(GRCh37). Ernst et al.(1987) stated that protein synthesis is inhibited due to phosphorylation of Eif2-alpha in hemin-deprived rabbit reticulocyte lysates. HeLa cells subjected to heat shock, serum deprivation, or interferon treatment followed by virus infection also show a correlation between EIF2-alpha phosphorylation and translational repression. Jacob et al.(1989) found that the alpha-Pal transcription factor bound to 2 palindromic sites within the EIF2-alpha promoter and was essential for transcription of the EIF2-alpha gene.
Synonyms: EIF 2 alpha antibody|EIF 2 antibody|EIF 2A antibody|EIF 2alpha antibody|eIF-2-alpha antibody|eIF-2A antibody|EIF-2alpha antibody|EIF2 alpha antibody|EIF2 antibody|EIF2A antibody|EIF2S1 antibody|Eukaryotic translation initiation factor 2 subunit 1 alpha 35 kDa antibody|Eukaryotic translation initiation factor 2 subunit 1 alpha antibody|Eukaryotic translation initiation factor 2 subunit 1 antibody|Eukaryotic translation initiation factor 2 subunit alpha antibody|IF2A_HUMAN antibody - UniProt
- P05198
- Pathways
- Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C
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