CD68 antibody (C-Term)

Catalog No. ABIN3044053

Product Details anti-CD68 Antibody

Target: CD68 (CD68 Molecule (CD68))

Binding Specificity: AA 341-354, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD68 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Macrosialin(CD68) detection. Tested with WB, IHC-P in Human.

Sequence: FCIIRRRPSA YQAL

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Macrosialin(CD68) detection. Tested with WB, IHC-P in Human.
Gene Name: CD68 Molecule
Protein Name: Macrosialin

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CD68(341-354aa FCIIRRRPSAYQAL).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for CD68 is approximately 1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-CD68 antibody (ABIN3044053)

Liu, Huang, Su, Song, Lu: "Blockage of Galectin-receptor Interactions by α-lactose Exacerbates Plasmodium berghei-induced Pulmonary Immunopathology." in: Scientific reports, Vol. 6, pp. 32024, (2018) (PubMed).

Yin, Chang, Xu: "Expressions Profiles of the Proteins Associated with Carbohydrate Metabolism in Rat Liver Regeneration." in: BioMed research international, Vol. 2017, pp. 8428926, (2018) (PubMed).

Li, Fang, Tian, Wang, Ao, Hou, Tong, Fan, Bai: "Curcumin attenuates the development of thoracic aortic aneurysm by inhibiting VEGF expression and inflammation." in: Molecular medicine reports, Vol. 16, Issue 4, pp. 4455-4462, (2018) (PubMed).

Chen, Shi, Liu, Ren, He, Chang, Yin: "Activation of corticotropin-releasing factor receptor 1 aggravates dextran sodium sulphate-induced colitis in mice by promoting M1 macrophage polarization." in: Molecular medicine reports, Vol. 17, Issue 1, pp. 234-242, (2018) (PubMed).

Hoffman, Adeli: "LDL Receptor Gene-Ablated Hamsters: A Rodent Model of Familial Hypercholesterolemia with Dominant Inheritance and Diet-Induced Coronary Atherosclerosis." in: EBioMedicine, Vol. 28, pp. 17-18, (2018) (PubMed).

Zhang, Liang, Zou, Chen, Shi, Wang, Zhang, Li, Zhai: "Inhibition of miR-155 attenuates abdominal aortic aneurysm in mice by regulating macrophage-mediated inflammation." in: Bioscience reports, Vol. 38, Issue 3, (2018) (PubMed).

Wang, Liu, Xiong, Xu, Shi, Qiu, Zang, Mao, Zhuang: "Inhibition of EGF Receptor Blocks the Development and Progression of Peritoneal Fibrosis." in: Journal of the American Society of Nephrology : JASN, Vol. 27, Issue 9, pp. 2631-44, (2017) (PubMed).

Liu, Xiao, Liu, Li, Zhou, Xian, Wang, Zhang, Wang, Ho, Zhuang: "SIV Infection Impairs the Central Nervous System in Chinese Rhesus Macaques." in: Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, Vol. 11, Issue 3, pp. 592-600, (2017) (PubMed).

Yang, Zhang, Yu, Tang, Gan: "Renin-angiotensin system activation accelerates atherosclerosis in experimental renal failure by promoting endoplasmic reticulum stress-related inflammation." in: International journal of molecular medicine, Vol. 39, Issue 3, pp. 613-621, (2017) (PubMed).

Li, Lu, Sun, Zuo, Wang, Yan: "Inhibition of endoplasmic reticulum stress signaling pathway: A new mechanism of statins to suppress the development of abdominal aortic aneurysm." in: PLoS ONE, Vol. 12, Issue 4, pp. e0174821, (2017) (PubMed).

Wang, Tian, Chen, Tan, Xie, Chen, Fu, Chen, Xu, Wei: "Low dose doxycycline decreases systemic inflammation and improves glycemic control, lipid profiles, and islet morphology and function in db/db mice." in: Scientific reports, Vol. 7, Issue 1, pp. 14707, (2017) (PubMed).

Wang, Zhang, Chai, Liu, Li, Wang, Zhu, Cui, Qu, Zhu: "1,25(OH)2D3 downregulates the Toll-like receptor 4-mediated inflammatory pathway and ameliorates liver injury in diabetic rats." in: Journal of endocrinological investigation, Vol. 38, Issue 10, pp. 1083-91, (2016) (PubMed).

Yan, Fan, Bai, Hou, Li, Tong: "Zinc Prevents Abdominal Aortic Aneurysm Formation by Induction of A20-Mediated Suppression of NF-κB Pathway." in: PLoS ONE, Vol. 11, Issue 2, pp. e0148536, (2016) (PubMed).

Zhang, Liu, Zhou, Hu, Qian: "Protective effect of eNOS overexpression against ischemia/reperfusion injury in small-for-size liver transplantation." in: Experimental and therapeutic medicine, Vol. 12, Issue 5, pp. 3181-3188, (2016) (PubMed).

Gu, Wu, Yao, Zeng, Shi, Lv, Luo, Song: "Intratracheal administration of mitochondrial DNA directly provokes lung inflammation through the TLR9-p38 MAPK pathway." in: Free radical biology & medicine, Vol. 83, pp. 149-58, (2015) (PubMed).

Peng, Liu, Wei, Lv, Wang, Xiong, Wang, Altaf, Wang, He, Wang, Qu: "P2X7R is involved in the progression of atherosclerosis by promoting NLRP3 inflammasome activation." in: International journal of molecular medicine, Vol. 35, Issue 5, pp. 1179-88, (2015) (PubMed).

Pan, Wang, Luo, Wang, Song, Ye, Hou: "Adipogenic changes of hepatocytes in a high-fat diet-induced fatty liver mice model and non-alcoholic fatty liver disease patients." in: Endocrine, Vol. 48, Issue 3, pp. 834-47, (2015) (PubMed).

Wang, Li, Zheng, Zhang, Peng, Zhang, Ai, Wang, Ma, Xiong, Li, Zhou, Shen: "Dynamic changes and functions of macrophages and M1/M2 subpopulations during ulcerative colitis-associated carcinogenesis in an AOM/DSS mouse model." in: Molecular medicine reports, Vol. 11, Issue 4, pp. 2397-406, (2015) (PubMed).

Liu, Xu, Huang, Wang, Gao, Li, Zhou, Ye, Chen, Jin, Liu: "Cryptotanshinone, an orally bioactive herbal compound from Danshen, attenuates atherosclerosis in apolipoprotein E-deficient mice: role of lectin-like oxidized LDL receptor-1 (LOX-1)." in: British journal of pharmacology, Vol. 172, Issue 23, pp. 5661-75, (2015) (PubMed).

He, Lv, Zhao, Xu, Jin, Qin, Yin: "Core-shell structured gel-nanocarriers for sustained drug release and enhanced antitumor effect." in: International journal of pharmaceutics, Vol. 484, Issue 1-2, pp. 163-71, (2015) (PubMed).

Target Details for CD68

Target: CD68 (CD68 Molecule (CD68))

Alternative Name: CD68 (CD68 Products)

Synonyms: Lamp4 antibody, Scard1 antibody, gp110 antibody, GP110 antibody, LAMP4 antibody, SCARD1 antibody, CD68 molecule antibody, CD68 antigen antibody, Cd68 molecule antibody, CD68 antibody, Cd68 antibody

Background: CD68, cluster of differentiation, is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. CD68 is a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34. The CD68 gene is mapped to 17p13.1. Immunohistochemistry can be used to identify the presence of CD68, which is found in the cytoplasmic granules of a range of different blood cells. It is particularly useful as a marker for the various cells of the macrophage lineage, including monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts. This allows it to be used to distinguish diseases of otherwise similar appearance, such as the monocyte/macrophage and lymphoid forms of leukaemia(the latter being CD68 negative). Its presence in macrophages also makes it useful in diagnosing conditions related to proliferation or abnormality of these cells, such as malignant histiocytosis, histiocytic lymphoma, and Gaucher's disease.

Synonyms: CD 68 antibody|CD68 antibody|CD68 antigen antibody|CD68 Molecule antibody|CD68_HUMAN antibody|DKFZp686M18236 antibody|GP110 antibody|LAMP4 antibody|Macrophage antigen CD68(microsialin) antibody|MACROPHAGE ANTIGEN CD68 antibody|macrosialin antibody|SCARD1 antibody|Scavenger receptor class D member 1 antibody

UniProt: P34810