HYAL1 antibody (N-Term)

Catalog No. ABIN3043999

Product Details anti-HYAL1 Antibody

Target: HYAL1 (Hyaluronidase-1 (HYAL1))

Binding Specificity: AA 32-47, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HYAL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for Hyaluronidase-1(HYAL1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human.

Sequence: FTTVWNANTQ WCLERH

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Hyaluronidase-1(HYAL1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human.
Gene Name: hyaluronoglucosaminidase 1
Protein Name: Hyaluronidase-1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human HYAL1(32-47aa FTTVWNANTQWCLERH).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HYAL1

Target: HYAL1 (Hyaluronidase-1 (HYAL1))

Alternative Name: HYAL1 (HYAL1 Products)

Synonyms: HYAL-1 antibody, LUCA1 antibody, NAT6 antibody, Hya1 antibody, Hyal-1 antibody, hyaluronoglucosaminidase 1 antibody, HYAL1 antibody, Hyal1 antibody

Background: Hyaluronidase-1, also known as HYAL1 or LUCA1, is an enzyme that in humans is encoded by the HYAL1 gene. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is though to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency.

Synonyms: HYAL 1 antibody|Hyal-1 antibody|HYAL1 antibody|HYAL1_HUMAN antibody|Hyaluronidase 1 antibody|Hyaluronidase-1 antibody|Hyaluronoglucosaminidase 1 antibody|Hyaluronoglucosaminidase-1 antibody|Hyaluronoglucosaminidase1 antibody|LUCA 1 antibody|LUCA-1 antibody|LUCA1 antibody|Lung carcinoma protein 1 antibody|MGC45987 antibody|NAT6 antibody|Plasma hyaluronidase antibody|Tumor suppressor LUCA 1 antibody

UniProt: Q12794

Pathways: Glycosaminoglycan Metabolic Process