EGR1 antibody (N-Term)

Catalog No. ABIN3043997

Product Details anti-EGR1 Antibody

Target: EGR1 (Early Growth Response 1 (EGR1))

Binding Specificity: AA 23-43, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for Early growth response protein 1(EGR1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.

Sequence: FPHSPTMDNY PKLEEMMLLS N

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Early growth response protein 1(EGR1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: early growth response 1
Protein Name: Early growth response protein 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Egr1(23-43aa FPHSPTMDNYPKLEEMMLLSN), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-EGR1 antibody (ABIN3043997)

Lin, Fang, Cai, Huang, Zhang, Lu, Zhang, Wei: "ERK/Egr-1 signaling pathway is involved in CysLT2 receptor-mediated IL-8 production in HEK293 cells." in: European journal of cell biology, Vol. 93, Issue 7, pp. 278-88, (2014) (PubMed).

Target Details for EGR1

Target: EGR1 (Early Growth Response 1 (EGR1))

Alternative Name: EGR1 (EGR1 Products)

Synonyms: AT225 antibody, G0S30 antibody, KROX-24 antibody, NGFI-A antibody, TIS8 antibody, ZIF-268 antibody, ZNF225 antibody, A530045N19Rik antibody, ETR103 antibody, Egr-1 antibody, Krox-1 antibody, Krox-24 antibody, Krox24 antibody, NGF1-A antibody, NGFIA antibody, Zenk antibody, Zfp-6 antibody, Zif268 antibody, egr antibody, Ngf1 antibody, Ngfi antibody, zif-268 antibody, EGR1 antibody, EGR-1-A antibody, Xegr-1 antibody, at225 antibody, egr-1 antibody, egr1 antibody, g0s30 antibody, krox-24 antibody, ngfi-a antibody, tis8 antibody, znf225 antibody, krox24 antibody, wu:fj64b05 antibody, wu:fq25f01 antibody, EGR-1 antibody, zenk antibody, EGR-1-B antibody, egr1-a antibody, egr1-b antibody, early growth response 1 antibody, early growth response protein 1 (egr-1) antibody, early growth response 1 L homeolog antibody, early growth response 1 S homeolog antibody, EGR1 antibody, CNK00890 antibody, Egr1 antibody, egr1.L antibody, egr1 antibody, egr1.S antibody

Background: Earlu growth response protein 1, also known as ZIF268 or NGFIA, is a protein that in humans is encoded by the EGR1 gene. The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. This gene is mapped to 5q31.2. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. It has a distinct pattern of expression in the brain, and its induction has been shown to be associated with neuronal activity. Several studies suggest it has a role in neuronal plasticity.

Synonyms: AT 225 antibody|AT225 antibody|Early growth response 1 antibody|Early growth response protein 1 antibody|EGR 1 antibody|EGR-1 antibody|EGR1 antibody|EGR1_HUMAN antibody|G0S 30 antibody|G0S30 antibody|KROX 24 antibody|Krox 24 protein antibody|KROX24 antibody|Nerve growth factor induced protein A antibody|Nerve growth factor-induced clone A antibody|Nerve growth factor-induced protein A antibody|NGFI A antibody|NGFI-A antibody|NGFIA antibody|TIS 8 antibody|TIS8 antibody|Transcription factor ETR 103 antibody|Transcription factor ETR103 antibody|Transcription factor Zif 268 antibody|Transcription factor Zif268 antibody|Zfp 6 antibody|ZIF 268 antibody|ZIF268 antibody|Zinc finger protein 225 antibody|Zinc finger protein Krox-24 antibody|ZNF 225 antibody|ZNF225 antibody

UniProt: P18146

Pathways: Regulation of Carbohydrate Metabolic Process, Regulation of long-term Neuronal Synaptic Plasticity