SLC12A2 antibody (N-Term)

Catalog No. ABIN3043994

The Rabbit Polyclonal anti-SLC12A2 antibody has been validated for WB, IHC and IF. It is suitable to detect SLC12A2 in samples from Human, Rat and Mouse.

Quick Overview for SLC12A2 antibody (N-Term) (ABIN3043994)

Target: SLC12A2 (Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 2 (SLC12A2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC12A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SLC12A2 Antibody

Binding Specificity: AA 223-241, N-Term

Purpose: Anti-NKCC1/SLC12A2 Antibody Picoband®

Sequence: RIDHYRHTAA QLGEKLLRP

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-NKCC1/SLC12A2 Antibody (ABIN3043994). Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human NKCC1, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunofluorescence, 5 μg/mL, Human
1. Dzhala, V. I., Talos, D. M., Sdrulla, D. A., Brumback, A. C., Mathews, G. C., Benke, T. A., Delpire, E., Jensen, F. E., Staley, K. J. NKCC1 transporter facilitates seizures in the developing brain. Nature Med. 11: 1205-1213, 2005. 2. Nguyen, M., Pace, A. J., Koller, B. H. Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia. J. Exp. Med. 204: 1383-1393, 2007.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SLC12A2

Target: SLC12A2 (Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 2 (SLC12A2))

Alternative Name: SLC12A2

Background:

Synonyms: Solute carrier family 12 member 2,Basolateral Na-K-Cl symporter,Bumetanide-sensitive sodium- (potassium)-chloride cotransporter 1,SLC12A2,NKCC1,

Tissue Specificity: Expressed in many tissues.

Background: Solute carrier family 12 (sodium/potassium/chloride transporters), member 2, also known as NKCC1, is widely distributed throughout the body, especially in organs that secrete fluids, called exocrine glands. By fluorescence in situ hybridization, this gene is mapped to chromosome 5q23.3. The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene.

Sequence Similarities: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

Molecular Weight: 200 kDa

UniProt: P55011

Pathways: Sensory Perception of Sound